FastQCFastQC Report
Fri 12 Nov 2021
HF2T7BGXK_n01_3012_d08_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2T7BGXK_n01_3012_d08_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998743
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTATGCCTTCTTCAAAAAAGCGCTCTAACTGATTGATGTCGTCATTGA60030.6010555267971841No Hit
ATTATATACGTCCCTTGCGTAAAGATTGTGTGATAGCTACTCTTTATTCT59740.598151876909275No Hit
GTGTAAGAAAAATGATTGTACTGTAAGCGCCTACATCTAATTTTCCTTCT53470.5353729638155161No Hit
CTGTGAGATAGAGTGGACCTAGTTTTCCTTGAACAAAAAAATTTCCGTAA42370.4242332612093402No Hit
GTACTAACAGTTCCACTAAATCTCCCTTTTTTGACTCATGAAATTCTAGC40330.40380758613577267No Hit
GTGAGATAGAGTGGACCTAGTTTTCCTTGAACAAAAAAATTTCCGTAAAT35480.355246544906948No Hit
ATATACGTCCCTTGCGTAAAGATTGTGTGATAGCTACTCTTTATTCTTTC35210.35254314673544646No Hit
GTCTAGGGTCTTTGTTAGAATACTTGTACTCCATCAAGTGGTGGCGTCTT34970.35014012613855616No Hit
AGTTTATAGACATCATGCCAGAAATTTTGTTGGGGCTAGCCATCAATACG32250.3229058927071328No Hit
ATACATGATGGGAAGCCATTGGGAAGATTTAAAGTAATGGATTACTCGGA31860.31900098423718615No Hit
CTTCTATGTGATTCTCTCAATGAGAGGATTGACTGTCAATAAATAAGCTC30890.30928877599142124No Hit
TCTCTGAAGCAAGCCTCATTAAGATTTATGGGCTTTGCAAATAATTCTGA29180.29216725423857787No Hit
GGCATAGATAGCATTATTGATCTTATCTCCTCTGTGCTACTGACTAGTAT29140.29176675080576286No Hit
GTGGTGGAACGAGAAGGCTCATGGCTAGCTGGATTGGGCTTCATAGGTCT28880.28916347849246504No Hit
CCATTATATACGTCCCTTGCGTAAAGATTGTGTGATAGCTACTCTTTATT28490.2852585700225183No Hit
GAGATAGAGTGGACCTAGTTTTCCTTGAACAAAAAAATTTCCGTAAATAG27700.2773486272244211No Hit
CTTGTACTCCATCAAGTGGTGGCGTCTTGCTCAACATTTGCAGCACGATT26750.2678366706950637No Hit
GGCTAATAGGAGGGAGAAGTGATTCTAGGCAAAGGGAAGCAGCAGCAAAG26100.2613284899118191No Hit
CCATAGACTATGGTTAGTAAAAACCCCCAGAGAACGGCAAAGCGCAAAAC26000.2603272313297815No Hit
CAGTAGCACAGAGGAGATAAGATCAATAATGCTATCTATGCCTTCTTCAA24980.2501143937929978No Hit
CCCCTACATGATAAAAATGCAAAAAAAGACCATACATCCTTTTTTTCAGT24470.24500797502460592No Hit
ACCATAGACTATGGTTAGTAAAAACCCCCAGAGAACGGCAAAGCGCAAAA24430.24460747159179086No Hit
GAGGAGATAAGATCAATAATGCTATCTATGCCTTCTTCAAAAAAGCGCTC24360.24390659058436456No Hit
TACTTGTACTCCATCAAGTGGTGGCGTCTTGCTCAACATTTGCAGCACGA24220.24250482856951186No Hit
CAATAATGCTATCTATGCCTTCTTCAAAAAAGCGCTCTAACTGATTGATG23910.23940092696519527No Hit
AGACTATGGTTAGTAAAAACCCCCAGAGAACGGCAAAGCGCAAAACAGGA23460.23489526334602595No Hit
TATTTAACCTGATCTTGAAAGTGTTATTTGATGGGCTAATAGGAGGGAGA23430.23459488577141469No Hit
CCATCAAGTGGTGGCGTCTTGCTCAACATTTGCAGCACGATTTCCGTATG22920.2294884670030228No Hit
ACATAAAGCTGAGCCAGATGTATTGTCTGGGTTAGAATTAATCTTTAGGC22840.22868746013739272No Hit
GCGTAAAGATTGTGTGATAGCTACTCTTTATTCTTTCCTTAACAAGTTTA22800.22828695670457766No Hit
TTTACGTACTGTGAGATAGAGTGGACCTAGTTTTCCTTGAACAAAAAAAT22310.22338078965259328No Hit
CTGTTAGTACAGATGCTTCATCCTTCTTATGTACGTGTTATCTGCAGATA21950.2197762587572579No Hit
CATTAAGATTTATGGGCTTTGCAAATAATTCTGAAAGTTTTTATGGAGCC21790.2181742450259977No Hit
TATCTCCTCTGTGCTACTGACTAGTATCACTTTTTTTCTACTTGCTCCCA21770.21797399330959016No Hit
TTATACGCCTGGCTGGCCCGTTCGATCTTTGTGGCCTCAAGCTGCTCAGC21610.21637197957832996No Hit
ATCCTGAAGAAACATTTATAAGCTTGTGGTTTAGTTGATATAATCATGGC21410.21436946241425472No Hit
ATTCAATAGTTGCTTGTTTAAAAAATTACCCTGCAAGGTATGAGAAACCA21100.21126556080993808No Hit
GTATGAGAAACCACCACCCTTGCTATGCCTCCCCTAGGGGAGGTTTGGTT20790.20816165920562146No Hit
GTAGTGGCTTATGGTCTAGGGTCTTTGTTAGAATACTTGTACTCCATCAA20720.20746077819819514No Hit
TTTATGGGCTTTGCAAATAATTCTGAAAGTTTTTATGGAGCCACAAGGTT20600.20625926789975No Hit
GTTTATAGACATCATGCCAGAAATTTTGTTGGGGCTAGCCATCAATACGG20530.20555838689232364No Hit
GGCTTATGGTCTAGGGTCTTTGTTAGAATACTTGTACTCCATCAAGTGGT19980.20005146469111676No Hit
CTAATGTATTACCAATATCTTTCTACATTCAAGTACAGAAGACATAAAGC19620.19644693379578132No Hit
GCACAGAGGAGATAAGATCAATAATGCTATCTATGCCTTCTTCAAAAAAG19460.19484492006452112No Hit
GGGTAGCATGACTAACAAAGCTATTTTGGGAGCAAGTAGAAAAAAAGTGA19450.19474479420631735No Hit
GTGTTGGATGTGGGTGGGTTAGGCGGAAGACTTAGCTTCTAAGGTTCGTG18850.18873724271409162No Hit
AGCTCGTACTTAGTGCACCCGCTTTTTTACGTACTGTGAGATAGAGTGGA18630.18653447383360885No Hit
GTAATGGATTACTCGGATGGTTAATAAAGCTAGAATTTCATGAGTCAAAA18580.18603384454259003No Hit
GGTCTAGGGTCTTTGTTAGAATACTTGTACTCCATCAAGTGGTGGCGTCT18440.18463208252773736No Hit
GATTTAAAGTAATGGATTACTCGGATGGTTAATAAAGCTAGAATTTCATG18400.18423157909492233No Hit
CTCTTTATTCTTTCCTTAACAAGTTTATAGACATCATGCCAGAAATTTTG17730.17752314659527027No Hit
ATTCTATACATGATGGGAAGCCATTGGGAAGATTTAAAGTAATGGATTAC17610.17632163629682512No Hit
ACATAGAAGTGACCAAGTCACCTGTTTATCATTTTCCTCAAACATGAGAT17480.1750200001401762No Hit
ATATAATCATGGCCGATTCAATAGTTGCTTGTTTAAAAAATTACCCTGCA17310.17331786055071224No Hit
ACCAATATCTTTCTACATTCAAGTACAGAAGACATAAAGCTGAGCCAGAT17240.17261697954328592No Hit
CCTCTGTGCTACTGACTAGTATCACTTTTTTTCTACTTGCTCCCAAAATA17090.17111509167022948No Hit
AGTCAGTAGCACAGAGGAGATAAGATCAATAATGCTATCTATGCCTTCTT17070.17091483995382195No Hit
AAACAGGAGAGACCAAGGCATGCCACCGAATGGCTCTAAAATTGGCTTCT16920.1694129520807655No Hit
GATCAATAATGCTATCTATGCCTTCTTCAAAAAAGCGCTCTAACTGATTG16840.16861194521513542No Hit
GTGCTACTGACTAGTATCACTTTTTTTCTACTTGCTCCCAAAATAGCTTT16720.16741043491669028No Hit
TTGCTGCACGTGCCTAAAAGCAGCCATACGGAAATCGTGCTGCAAATGTT16670.16690980562567148No Hit
GGAATGGGTAGCATGACTAACAAAGCTATTTTGGGAGCAAGTAGAAAAAA16450.16470703674518872No Hit
TTCAAGTACAGAAGACATAAAGCTGAGCCAGATGTATTGTCTGGGTTAGA16140.16160313514087207No Hit
GTATATAATGGAGCAACTGAAAAAAAGGATGTATGGTCTTTTTTTGCATT16130.1615030092826683No Hit
ATACTAGTCAGTAGCACAGAGGAGATAAGATCAATAATGCTATCTATGCC16080.16100237999164949No Hit
TCTCTATACTGGTATTGTTAGTGTCTCACTTGAAATGAACCTGTTGATAA15870.1588997369693705No Hit
GTAAGCGCCTACATCTAATTTTCCTTCTACAACAAGAAACCCACCATAGA15870.1588997369693705No Hit
GTCTAAATGACGGCTCTCCTACTAAGTCCTGCTGCAGAGAATACATGCTG15850.15869948525296299No Hit
CTATACATGATGGGAAGCCATTGGGAAGATTTAAAGTAATGGATTACTCG15620.15639659051427643No Hit
GTGCAGCAAAGTACGATGGCTTCTTTTTCCAAACAAAAAACTGGCAATTT15570.15589596122325763No Hit
AGCAGGCGCTGTGTAAGAAAAATGATTGTACTGTAAGCGCCTACATCTAA15510.15529520607403507No Hit
GACCAAGGCATGCCACCGAATGGCTCTAAAATTGGCTTCTTTATACGCCT15300.15319256305175608No Hit
CTATTGGCCAAGCTCGTACTTAGTGCACCCGCTTTTTTACGTACTGTGAG15290.15309243719355228No Hit
GTAAACTATTGGCCAAGCTCGTACTTAGTGCACCCGCTTTTTTACGTACT15210.1522914303279222No Hit
GATGTATTGTCTGGGTTAGAATTAATCTTTAGGCAGGCAGATGGCTTTTT14990.15008866144743943No Hit
CAATACGGTGGTGGAACGAGAAGGCTCATGGCTAGCTGGATTGGGCTTCA14760.1477857667087529No Hit
GGCTAAGGGATTTGAATTTTATTCTATACATGATGGGAAGCCATTGGGAA14540.14558299782827014No Hit
CTCGTACTTAGTGCACCCGCTTTTTTACGTACTGTGAGATAGAGTGGACC14350.14368060652239864No Hit
GTGTATAGTTTAGATGTCAAACTTCCTCTCTATACTGGTATTGTTAGTGT14290.14307985137317608No Hit
TTATTGATCTTATCTCCTCTGTGCTACTGACTAGTATCACTTTTTTTCTA14240.1425792220821573No Hit
GCATTATTGATCTTATCTCCTCTGTGCTACTGACTAGTATCACTTTTTTT14110.14127758592550835No Hit
TCTTTATACGCCTGGCTGGCCCGTTCGATCTTTGTGGCCTCAAGCTGCTC13990.1400760756270632No Hit
GTATCAATGCATAGTGAGCTATCCTGAAGAAACATTTATAAGCTTGTGGT13980.13997594976885946No Hit
GGTTAATAAAGCTAGAATTTCATGAGTCAAAAAAGGGAGATTTAGTGGAA13940.1395754463360444No Hit
TTCCACCACCGTATTGATGGCTAGCCCCAACAAAATTTCTGGCATGATGT13900.13917494290322938No Hit
ATCAAGTGGTGGCGTCTTGCTCAACATTTGCAGCACGATTTCCGTATGGC13810.13827381017939552No Hit
GGATTACTCGGATGGTTAATAAAGCTAGAATTTCATGAGTCAAAAAAGGG13800.13817368432119173No Hit
TGCTATGCCTCCCCTAGGGGAGGTTTGGTTTTTTTGGCACTTGTTCATCT13360.1337681465602262No Hit
GCTTTTAGGCACGTGCAGCAAAGTACGATGGCTTCTTTTTCCAAACAAAA13360.1337681465602262No Hit
GTGTGATAGCTACTCTTTATTCTTTCCTTAACAAGTTTATAGACATCATG13320.13336764312741115No Hit
GCTAATAGGAGGGAGAAGTGATTCTAGGCAAAGGGAAGCAGCAGCAAAGG13220.13236638454537353No Hit
TGTTAGTACAGATGCTTCATCCTTCTTATGTACGTGTTATCTGCAGATAA13020.1303638673812983No Hit
ATCTCACAGTACGTAAAAAAGCGGGTGCACTAAGTACGAGCTTGGCCAAT12900.12916235708285315No Hit
CTGTAGTGGCTTATGGTCTAGGGTCTTTGTTAGAATACTTGTACTCCATC12890.12906223122464938No Hit
CTTTATACGCCTGGCTGGCCCGTTCGATCTTTGTGGCCTCAAGCTGCTCA12870.12886197950824188No Hit
TTATTAGGCTAAGGGATTTGAATTTTATTCTATACATGATGGGAAGCCAT12840.1285616019336306No Hit
GTACTGTAAGCGCCTACATCTAATTTTCCTTCTACAACAAGAAACCCACC12790.12806097264261176No Hit
GACTAGTATCACTTTTTTTCTACTTGCTCCCAAAATAGCTTTGTTAGTCA12750.12766046920979673No Hit
ATGATAAACAGGTGACTTGGTCACTTCTATGTGATTCTCTCAATGAGAGG12750.12766046920979673No Hit
TGATTGTACTGTAAGCGCCTACATCTAATTTTCCTTCTACAACAAGAAAC12700.1271598399187779No Hit
GTAGCACAGAGGAGATAAGATCAATAATGCTATCTATGCCTTCTTCAAAA12590.1260584554785365No Hit
CCCTAGGGGAGGTTTGGTTTTTTTGGCACTTGTTCATCTAGTATTTTTGC12530.12545770032931394No Hit
GCCTCAAGCTGCTCAGCTACTAAACTCTTGATGGTAAGCATGCCCAGTAA12300.12315480559062743No Hit
GATCTTGAAAGTGTTATTTGATGGGCTAATAGGAGGGAGAAGTGATTCTA12210.12225367286679356No Hit
CTATTAAGATCCTGTAAATGACATACAGCCAGGTGTATTGTTATTTCGTC12080.12095203671014464No Hit
AGTCAAAGGAATGGGTAGCATGACTAACAAAGCTATTTTGGGAGCAAGTA11950.11965040055349574No Hit
ATGCTATCTATGCCTTCTTCAAAAAAGCGCTCTAACTGATTGATGTCGTC11940.11955027469529199No Hit
CTATACTGGTATTGTTAGTGTCTCACTTGAAATGAACCTGTTGATAAGCT11930.11945014883708822No Hit
AACTGATTGATGTCGTCATTGAGAATGGAAAGCAAATTGCCAGTTTTTTG11890.11904964540427317No Hit
CTTTAAAACATCCAATCTCATGTTTGAGGAAAATGATAAACAGGTGACTT11820.11834876439684684No Hit
ATAGTGAGCTATCCTGAAGAAACATTTATAAGCTTGTGGTTTAGTTGATA11730.11744763167301298No Hit
GACTATGGTTAGTAAAAACCCCCAGAGAACGGCAAAGCGCAAAACAGGAG11690.11704712824019793No Hit
GAATAAAGAGTAGCTATCACACAATCTTTACGCAAGGGACGTATATAATG11670.1168468765237904No Hit
CTATAAACTTGTTAAGGAAAGAATAAAGAGTAGCTATCACACAATCTTTA11610.11624612137456783No Hit
CCCCCAGAGAACGGCAAAGCGCAAAACAGGAGAGACCAAGGCATGCCACC11530.11544511450893773No Hit
CCCTAGACCATAAGCCACTACAGTCACTACCCCCAGCAAAAGCATCTGTA11520.11534498865073398No Hit
GTACGATGGCTTCTTTTTCCAAACAAAAAACTGGCAATTTGCTTTCCATT11490.11504461107612268No Hit
TTTATAAGCTTGTGGTTTAGTTGATATAATCATGGCCGATTCAATAGTTG11460.1147442335015114No Hit
ACCCTAGACCATAAGCCACTACAGTCACTACCCCCAGCAAAAGCATCTGT11450.11464410764330764No Hit
CATAAGAAGGATGAAGCATCTGTACTAACAGTTCCACTAAATCTCCCTTT11410.11424360421049258No Hit
GTGCATCCTCAGGATAAAAAGATAAAGATAAACCTGAGCAGTCTAAATGA11340.11354272320306626No Hit
ATACGGTGGTGGAACGAGAAGGCTCATGGCTAGCTGGATTGGGCTTCATA11260.11274171633743615No Hit
TTTTTACGTACTGTGAGATAGAGTGGACCTAGTTTTCCTTGAACAAAAAA11220.11234121290462111No Hit
TGGCTGGCCCGTTCGATCTTTGTGGCCTCAAGCTGCTCAGCTACTAAACT11190.11204083533000982No Hit
GAGTGGACCTAGTTTTCCTTGAACAAAAAAATTTCCGTAAATAGTCAAAG11160.11174045775539854No Hit
CCATAAGCCACTACAGTCACTACCCCCAGCAAAAGCATCTGTATTTTTAG11100.11113970260617596No Hit
GTCAAATATTGAAGCAAAGTTTTAGATTAATGTGTTAGATAGCATTGCCA11040.11053894745695339No Hit
GATTGATGTCGTCATTGAGAATGGAAAGCAAATTGCCAGTTTTTTGTTTG11000.11013844402413835No Hit
GGGTAGTGACTGTAGTGGCTTATGGTCTAGGGTCTTTGTTAGAATACTTG10960.1097379405913233No Hit
GAGTACAAGTATTCTAACAAAGACCCTAGACCATAAGCCACTACAGTCAC10960.1097379405913233No Hit
GTACAGAAGACATAAAGCTGAGCCAGATGTATTGTCTGGGTTAGAATTAA10920.10933743715850824No Hit
GTTAGAATACTTGTACTCCATCAAGTGGTGGCGTCTTGCTCAACATTTGC10900.10913718544210073No Hit
GCAAAGTACGATGGCTTCTTTTTCCAAACAAAAAACTGGCAATTTGCTTT10900.10913718544210073No Hit
ATATAATGGAGCAACTGAAAAAAAGGATGTATGGTCTTTTTTTGCATTTT10890.10903705958389695No Hit
CTAAAAATACAGATGCTTTTGCTGGGGGTAGTGACTGTAGTGGCTTATGG10860.10873668200928567No Hit
CCCTTGCTATGCCTCCCCTAGGGGAGGTTTGGTTTTTTTGGCACTTGTTC10700.10713466827802548No Hit
ATTCTAGGCAAAGGGAAGCAGCAGCAAAGGCTCGGAGGTAGGAAAAAGAA10690.10703454241982173No Hit
GTATTGTCTGGGTTAGAATTAATCTTTAGGCAGGCAGATGGCTTTTTATT10680.10693441656161795No Hit
GTAAAAACCCCCAGAGAACGGCAAAGCGCAAAACAGGAGAGACCAAGGCA10650.10663403898700666No Hit
CTCTAACTGATTGATGTCGTCATTGAGAATGGAAAGCAAATTGCCAGTTT10500.10513215111395023No Hit
CCGTATGGCTGCTTTTAGGCACGTGCAGCAAAGTACGATGGCTTCTTTTT10390.10403076667370886No Hit
CTTCAAAAAAGCGCTCTAACTGATTGATGTCGTCATTGAGAATGGAAAGC10370.10383051495730132No Hit
TCTTTATTCTTTCCTTAACAAGTTTATAGACATCATGCCAGAAATTTTGT10340.10353013738269004No Hit
GAATTAATCTTTAGGCAGGCAGATGGCTTTTTATTTTTAAACAATTTATG10220.1023286270842449No Hit
CTTTTAGGCACGTGCAGCAAAGTACGATGGCTTCTTTTTCCAAACAAAAA10220.1023286270842449No Hit
GTTTATATTTAACCTGATCTTGAAAGTGTTATTTGATGGGCTAATAGGAG10120.10132736850220728No Hit
GATAAGATCAATAATGCTATCTATGCCTTCTTCAAAAAAGCGCTCTAACT10070.10082673921118845No Hit
GTAGGTAGGGTGGTGGTCATCAACAATTTATTAGGCTAAGGGATTTGAAT10030.10042623577837342No Hit
CTGTAAGCGCCTACATCTAATTTTCCTTCTACAACAAGAAACCCACCATA9990.10002573234555838No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)