FastQCFastQC Report
Fri 12 Nov 2021
HF2T7BGXK_n01_2890_d06_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2T7BGXK_n01_2890_d06_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4148269
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTC364540.8787761835117249No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCA304950.7351259043229839No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG265220.6393510160503092No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA186830.45038062864293515No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT181730.43808634396660395No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACG165020.39780448182121264No Hit
GAGCAAAAGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCT160420.3867155191719727No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA144350.3479764692212583No Hit
GTACGGATAACAAATAGCAGCACTGCTATAACTATTTTAATGCATGTGTC122680.29573781256712134No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG119530.2881442837964462No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT117960.2843595726313795No Hit
GCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGG117850.2840944017854194No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG116260.28026147773926907No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT100510.24229383388589312No Hit
CATTAGCCTTTTCCCCCTTAGCAAGGTTACTCAGTTCATTGATGCTTAAT98070.23641186239368758No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG94610.22807103396621578No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC88320.21290808286540722No Hit
GAATAAAAGAACTTCGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA88000.2121366767680688No Hit
CAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCG85700.2065921954434488No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA81740.19704604498888573No Hit
AGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCTTCAATCC81610.19673266126184197No Hit
CCCTAGAAGGTCCGGGGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA79690.1921042246778114No Hit
AGCGAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCG78730.1897900063857961No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG78610.1895007290992942No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT78050.18815076842895193No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA71860.17322888173356163No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC71450.17224051767134677No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG70710.17045664107125166No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA68900.16609337533318114No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG68510.16515322415204992No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT67020.16156136451131786No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA65080.1568847150462036No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG64620.1557758187812796No Hit
ATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCAGTC64120.15457049675418832No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC60570.14601271036184008No Hit
TTTTTGGACAGTACGGATAACAAATAGCAGCACTGCTATAACTATTTTAA60410.14562700731317088No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG58770.1416735510643114No Hit
GGTATGGGGAGGATCTGAAAATTGAAACTAACAAATTTGCAGCAATATGC58490.1409985707291403No Hit
GGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATG58000.1398173551425908No Hit
AGCTAATAGAGTCCTACAGACTTTTCCAATGGAACCTTCTTCCACTCCTT57150.13776830769653559No Hit
ATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT56270.1356469409288549No Hit
GTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGCGTTTCCTGGG55760.1344175124612218No Hit
ACAAATAGCAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAG54500.1313801009529517No Hit
GTTAAAGCACAGATTTGAAATAATCGAGGGGAGAGACAGAACAATGGCCT54160.1305604819745296No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA54110.1304399497718205No Hit
TATCAAAAGAGGGCACGATCGGGTTCGCTGCCTTCTCGTCTGAGAGCTCG53780.12964443723394023No Hit
GTCTCGCACTCGCGAGATACTGACAAAAACCACAGTGGACCATATGGCCA53740.12954801147177294No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC52980.12771592199059414No Hit
ATTCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA52380.1262695355580846No Hit
TAGCAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA52010.12537759725803704No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCA51750.12475082980394955No Hit
TCTCAGGACGGCGGACTCCACTCCGGATGTGCTTTCATCTGGGTCTTCAA51510.12417227523094573No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC49440.11918224203878774No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCG49330.11891707119282766No Hit
ATGTTCCTGAGAGAAATGAACAAGGACAAACTCTATGGAGTAAAATGAGT48290.11641000137647775No Hit
AGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCG48250.11631357561431045No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGA48180.11614483053051768No Hit
CCATATGGCCATAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACC48130.11602429832780854No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG47390.11424042172771341No Hit
TGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACGATCAAGAATCCA47140.11363776071416777No Hit
ATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGAT47120.11358954783308411No Hit
CAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGAGT47070.11346901563037497No Hit
GCAAAAGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCTTC46780.11276992885466203No Hit
CATTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCATCAATGAACA46760.11272171597357838No Hit
GAGCGAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTC46360.11175745835190534No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA45610.10994947531126839No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT45520.10973251734639196No Hit
GGTTTGGCCTGCGGAGGCCCTCTGTTGATTAGTGTTTCCTCCACTCCTGG45060.10862362108146795No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATTCTATTGTATA45000.108478982438217No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT44830.10806917294900596No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC44770.107924534305755No Hit
GTATACATTGTGGTCATAAGTTCCATTCCTGATTGACCCCATGCAGGCAT43650.10522461296507049No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGTTTGTCCCT43070.10382643941364458No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA42630.10276575602980423No Hit
TTCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAA42620.10274164958926242No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT42600.10269343670817876No Hit
CTCAGTATCAGTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC42350.10209077569463311No Hit
GCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCC42250.10184971128921484No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG41810.10078902790537451No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTC41750.10064438926212355No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)