FastQCFastQC Report
Fri 12 Nov 2021
HF2T7BGXK_n01_2882_d04_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2T7BGXK_n01_2882_d04_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences851455
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACG52010.6108367441614647No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT36960.434080485756734No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA25310.2972558737690189No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG24310.28551127188166137No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT23590.27705515852276397No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC23210.27259220980556814No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG22510.26437098848441787No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT19800.23254311736967898No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC19420.22808016865248312No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG19230.22584869429388515No Hit
CCCTAGAAGGTCCGGGGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA18510.21739258093498776No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCA17750.20846668350059605No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC16980.19942334004733075No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG15220.1787528407255815No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA15190.17840050266896076No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT14860.1745247840461328No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA14840.17428989200838566No Hit
GTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGCGTTTCCTGGG14560.17100140347992554No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT13970.1640720883663846No Hit
CTCATAGGCAGATGGTGGCAACAACCAATCCATTAATAAAACATGAGAAC13930.1636023042908903No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC13650.1603138157624302No Hit
TGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACGATCAAGAATCCA12960.1522100404601535No Hit
ATTCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA12840.1508006882336706No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG12690.14903899795056696No Hit
CATGAGAACAGAATGGTTTTGGCCAGCACTACAGCTAAGGCTATGGAGCA12630.14833432183732553No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA12050.14152245274265818No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA11850.13917353236518665No Hit
GTCCTGAATTCTCCCTTCTACTTCTGAGAACTCTTTTTCAATCTGATGGA11840.13905608634631308No Hit
GCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCC11740.13788162615757732No Hit
GGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTCTCATA11730.13776418013870376No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG11650.13682461198771514No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT11600.13623738189334728No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCG11420.13412335355362293No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT11410.13400590753474934No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG11250.13212677123277214No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC11040.12966040483642705No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC10870.1276638225155763No Hit
ATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT10770.12648936232684052No Hit
CAATAAGCGGATCACTTGAATCGTTGCATCTGCACCCCCATTCGTTTCTG10640.12496256408148405No Hit
CAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGAGT10460.12284853574175969No Hit
CTCAGTATCAGTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC10360.12167407555302395No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA10340.12143918351527679No Hit
CCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGAGTCGACAGACGCAT10320.12120429147752965No Hit
ATCCCAATGATATTCGCGGCAACAATAAGCGGATCACTTGAATCGTTGCA10120.11885537110005813No Hit
CGTATGTTCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCG10060.11815069498681668No Hit
TATCAAAAGAGGGCACGATCGGGTTCGCTGCCTTCTCGTCTGAGAGCTCG9910.11638900470371306No Hit
TTCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAA9820.11533199053385089No Hit
CCACAATATCAAGTGCAAGATCCCAATGATATTCGCGGCAACAATAAGCG9710.11404008432624156No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAATA9600.11274817811863223No Hit
ATCAAGTGCAAGATCCCAATGATATTCGCGGCAACAATAAGCGGATCACT9430.11075159579778143No Hit
ACAATAAGCGGATCACTTGAATCGTTGCATCTGCACCCCCATTCGTTTCT9360.10992947366566642No Hit
CTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAA9330.1095771356090457No Hit
GGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAATAAAACATGAG9280.10898990551467781No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT9250.1086375674580571No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA9180.10781544532594206No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATTCTATTGTATA8970.10534907892959698No Hit
TCTGATAGGTCTGCAAATTTTCAAGAAGATCATCTCTTAGACCAGTACTG8930.10487929485410268No Hit
GTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTG8890.10440951077860837No Hit
CATACGGGGCATGTCCCAGATATGTTAAGCAAAGCACTCTGAAATTGGCA8840.1038222806842405No Hit
GCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCAGCGGAGGC8740.10264782049550475No Hit
GTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTCTCATAGGCAG8680.1019431443822633No Hit
ATTGTATGTTGGTTCTCCAGGGCAACAAGAAGCTCCGCGTTGTATGACCA8660.10170825234451615No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCATGGAACGTTATCTCCCTCTT8630.10135591428789543No Hit
CTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCAGCG8540.10029890011803325No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)