FastQCFastQC Report
Fri 12 Nov 2021
HF2T7BGXK_n01_2856_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2T7BGXK_n01_2856_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2572503
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG92730.3604660519346333No Hit
GGATAAGACGCGCCAGCGTCGCATCCGGTATTAATTGATATGCAAAAAAC90020.3499315647056583No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA69840.27148656386406544No Hit
GGAGCAAACGCGCCAGCGTCGCATCCGGCATTTATTCCGCACCAACGCAA59290.2304759216996054No Hit
GTATTAATTGATATGCAAAAAACCCCGGCCTTATTGACCGGGGTTTTTCT59160.22997057729378742No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT57800.2246838973559992No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG53130.2065303713931529No Hit
GTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGCGTTGT48800.18969851541475363No Hit
GAGCAAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATC47130.18320678343232252No Hit
GTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGC43990.17100077239948797No Hit
CGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTG40810.15863927078024787No Hit
CATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTA40170.1561514213977593No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATA39670.15420778906769012No Hit
GAGCAAAAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAG39540.1537024446618721No Hit
AATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCT36860.1432845753727012No Hit
GTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTT36640.14242937714747078No Hit
GTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGA35060.1362874989844521No Hit
TCATTAACCCGCTTGTCGCCGGTTAATCTAATCTGTATCAGGCTGAAAAT31250.12147702062932483No Hit
CATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAA30710.1193778977128501No Hit
AATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGC30670.11922240712644455No Hit
GGAGCAAACGCGCCAGCGTCGCATCCGGTATTAATTGATATGCAAAAAAC30390.1181339730216058No Hit
ATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGT30100.11700666627016568No Hit
GGATAAGACGCGCCAGCGTCGCATCCGGCATTTATTCCGCACCAACGCAA28390.11035944370132902No Hit
GGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCG26780.1041009475985062No Hit
GGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCAT26240.10200182468203148No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)