FastQCFastQC Report
Fri 12 Nov 2021
HF2T7BGXK_n01_2855_d04.2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2T7BGXK_n01_2855_d04.2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2259654
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACG138020.6108014766862537No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT108980.4822862261213442No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA77460.34279584396549206No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG68710.30407310145712574No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT63600.2814590198322398No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG63580.28137051070650637No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG53540.2369389295883352No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG52900.23410663756486613No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC50420.22313150597392345No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT48970.2167145943582513No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT48860.21622779416671756No Hit
CCCTAGAAGGTCCGGGGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA48310.21379379320904882No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA46650.20644753577317587No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA44840.19843745989430242No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCA43490.19246309390729732No Hit
ATTCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA39690.17564636001794967No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC39640.17542508720361613No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC39210.17352214100034782No Hit
ATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT37720.1669282111332089No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT36000.15931642632013573No Hit
GTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGCGTTTCCTGGG35910.1589181352543354No Hit
CTCATAGGCAGATGGTGGCAACAACCAATCCATTAATAAAACATGAGAAC35860.15869686244000186No Hit
TGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACGATCAAGAATCCA35310.15626286148233312No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG34950.1546696972191318No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG32840.14533198445425716No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC32580.14418136581972285No Hit
GCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCC32570.14413711125685613No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA32320.14303074718518852No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT32150.14227841961645457No Hit
GGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTCTCATA31860.14099503729332014No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC31460.13922485477865196No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCG31210.13811849070698434No Hit
GAATAAGGGATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA30680.135772998875049No Hit
GTCCTGAATTCTCCCTTCTACTTCTGAGAACTCTTTTTCAATCTGATGGA30540.13515343499491514No Hit
CATGAGAACAGAATGGTTTTGGCCAGCACTACAGCTAAGGCTATGGAGCA30400.1345338711147813No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC30150.13342750704311368No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA29910.13236539753431278No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT29530.13068372414537802No Hit
GTATACATTGTGGTCATAAGTTCCATTCCTGATTGACCCCATGCAGGCAT28910.12793994124764232No Hit
CAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGAGT28610.1266123043616412No Hit
CAATAAGCGGATCACTTGAATCGTTGCATCTGCACCCCCATTCGTTTCTG28440.12585997679290722No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG28200.12479786728410633No Hit
CTCAGTATCAGTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC28010.12395703058963896No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA27580.12205408438637065No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA27540.12187706613490383No Hit
TATCAAAAGAGGGCACGATCGGGTTCGCTGCCTTCTCGTCTGAGAGCTCG26830.11873499217136783No Hit
CCCTTATTCTGGGTCTAGATCCGATATTCGGGATTACAGCTTGTTGGCTT26650.11793841003976714No Hit
ATCCCAATGATATTCGCGGCAACAATAAGCGGATCACTTGAATCGTTGCA26610.11776139178830035No Hit
CGTATGTTCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCG26520.1173631007225No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAATA26280.11630099121369909No Hit
TTCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAA26240.11612397296223227No Hit
ATCAAGTGCAAGATCCCAATGATATTCGCGGCAACAATAAGCGGATCACT26220.11603546383649886No Hit
CCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGAGTCGACAGACGCAT26090.1154601545192317No Hit
CTCATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCT25940.11479633607623115No Hit
CATACGGGGCATGTCCCAGATATGTTAAGCAAAGCACTCTGAAATTGGCA25830.11430953588469739No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATTCTATTGTATA25430.11253935337002922No Hit
GTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTCTCATAGGCAG25100.11107895279542797No Hit
ATTGTATGTTGGTTCTCCAGGGCAACAAGAAGCTCCGCGTTGTATGACCA25040.11081342541822774No Hit
GGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAATAAAACATGAG24940.11037087978956071No Hit
ACAATAAGCGGATCACTTGAATCGTTGCATCTGCACCCCCATTCGTTTCT24500.1084236790234257No Hit
CCACAATATCAAGTGCAAGATCCCAATGATATTCGCGGCAACAATAAGCG24250.1073173149517581No Hit
CTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACT24230.10722880582602469No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC24120.10674200563449093No Hit
CTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGA23640.10461778661688913No Hit
ATGTTAAGCAAAGCACTCTGAAATTGGCAACAGGAATGCGAAATGTACCA23570.10430800467682219No Hit
GTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTG23450.10377694992242176No Hit
GCCTTAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAA23450.10377694992242176No Hit
GTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCAC23370.10342291341948812No Hit
CTATTGGACAATAGTAAAACCAGGAGACATACTTTTGATTAACAGCACAG23330.1032458951680213No Hit
CTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAA23000.10178549459342004No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC22630.10014807576735199No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)