FastQCFastQC Report
Fri 7 Aug 2020
HF2CNAFX2_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2CNAFX2_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9019840
Sequences flagged as poor quality0
Sequence length76
%GC65

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG174702319.368669510767376No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG198680.22026998261609962No Hit
GGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG111740.12388246354702523No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG103280.11450313974527265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC2550.024.73779149
GCCGTAT2600.024.2616648
CGCCGTA2551.8189894E-1220.6113847
CGTATCA3200.019.71303650
TCTCGCG3001.0164513E-815.1208547
TCGCCGT3801.035005E-913.83116646
TTCGGGC7900.013.6909446
TCTCGGT4103.2250682E-912.81777237
TTGGGCG19200.012.3583917
CTCCCGT4551.2278178E-912.2699718
GATCTCG4306.5720087E-912.21900935
TAGTTCG2356.9723313E-411.8907163
GTTTCGG7750.011.7011435
CGCTACG2701.7222352E-411.6314277
TCGGGCG18250.011.4720937
TAACGGC2751.9621183E-411.46940369
TTTCGGC3054.2049363E-511.4392876
TCGTGTA6750.011.38078115
GTAGATC4954.3528416E-911.32067632
TACCCTA6006.730261E-1111.0495499
Produced by FastQC (version 0.11.5)