FastQCFastQC Report
Fri 7 Aug 2020
HF2CNAFX2_n02_RPE-1_WT_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2CNAFX2_n02_RPE-1_WT_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13685615
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1569521.146839217674909No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGT20800.017.1647114
CGACCAT43600.014.7717184
TGGCGCG24550.014.5503123
GACGACC50150.013.6204222
CGCGTGC27400.013.4057886
ACGACCA48550.013.3446173
TCGGCAT59500.012.94495969
CGTAGGG18450.012.90236764
GTCGTGA24100.012.62787915
GGCGATC15100.012.5269032
AGTTCGG62050.012.46871366
CCGATCG62150.012.44701146
GCGCGTG29400.012.3760175
GTGTCGT25550.012.18821113
CCCGATA53250.012.1549112
GGGCGAT16150.012.1481221
CGCACTA62200.012.10240459
GTTCGGC63100.012.03948467
TTCGTCG15700.012.039268538
ATCGGGT63750.011.91781849
Produced by FastQC (version 0.11.5)