FastQCFastQC Report
Fri 7 Aug 2020
HF2CNAFX2_n02_RPE-1_WT_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2CNAFX2_n02_RPE-1_WT_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8333050
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1544351.853283011622395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGT19350.019.3518854
TGGCGCG19600.019.1142313
CGCGTGC23650.015.8253796
GCGCGTG25300.014.7945325
CGACCAT23450.014.4760274
GTGGCGC26000.014.4133452
TAGGTCG3655.711627E-1014.3888993
CGGTCGA7400.014.18516427
TCGGCAT45900.013.95858769
GGCGATC8000.013.57144452
CGCACTA48650.013.1705159
ATTGTCG13850.013.144870
GTTCGGC49400.013.11112667
ACGACCA25550.013.0185273
GGTGGCG28900.012.9696731
CTTGACG7900.012.8592011
AGTTCGG49700.012.820566
ATCGGGT50300.012.80754649
GCGTCCT14000.012.74806933
GTCGTGA14550.012.74170115
Produced by FastQC (version 0.11.5)