FastQCFastQC Report
Fri 7 Aug 2020
HF2CNAFX2_n02_RPE-1_WT_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2CNAFX2_n02_RPE-1_WT_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6401830
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG719111.1232881847846632No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAC1704.376959E-516.47671159
GGCGCGT8150.015.4599484
TCGCGTT1605.159178E-415.31543838
CGCGTTT2403.4464501E-614.58635939
GGCGATC6950.014.1128042
GTCGCGT1800.001258622313.61361837
CGACCAT20800.012.6200494
CGTAGGG8050.012.611608564
GACGACC21400.012.6042512
CCGATCG24500.012.43066546
CCGGTCG2305.703288E-412.17824851
TTGCCGC7200.012.1589133
GGGCGAT7800.012.1282351
CCCGATA22050.012.05760612
CGCGTGC11050.012.0289166
CGCACTA25000.011.90442559
ACGACCA23000.011.5710543
TGGCGCG11200.011.56833653
ATCGGGT26500.011.49456849
CGGTCGC2150.004767856611.39940752
Produced by FastQC (version 0.11.5)