FastQCFastQC Report
Fri 7 Aug 2020
HF2CNAFX2_n02_RPE-1_WT_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2CNAFX2_n02_RPE-1_WT_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7510868
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG950561.2655794243754517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG10400.019.52367864
CTTGACG7450.018.8092611
GGTAGTC12500.015.96501869
CGACCAT17650.014.489261
AACGTAG14700.014.05081862
GTAGGGT14900.013.62726565
GGCGCGT7000.013.4989384
GGGTAGT15350.013.228826568
GACGACC17000.013.1862372
GAACGTA17850.012.747745561
TCGGCAT21200.012.71626169
CCGATCG21050.012.63886846
ACGACCA17250.012.1794443
CGATCGG23600.012.01480947
CGCACTA21750.011.91210859
ATCGGGT22200.011.82812249
GTTCGGC22900.011.77202467
CGCGTGC8550.011.4557456
GTCCGCA23300.011.42088956
TCGCAAT8300.011.3871540
Produced by FastQC (version 0.11.5)