FastQCFastQC Report
Fri 7 Aug 2020
HF2CNAFX2_n02_N2N3N1KO_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2CNAFX2_n02_N2N3N1KO_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5273265
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG601151.1399958090480946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG17700.025.3007379
TAACGGG4550.023.08488869
CTCGTAT7950.022.88686230
CGTATAA8150.022.75649632
ACTAACG4950.022.63192467
ATATCGC20050.022.3353148
CTAACGG4950.021.92571668
ATTCGCG5300.021.78095218
ATCGCGG20900.021.4269410
TCGTATA8550.021.28197731
CGCTAAT8300.021.07780824
TACGTTC8500.020.99170510
CGACGTA6350.020.38722
CGCTCGA6350.020.38293818
CTCGACG6550.020.29578220
TCGACGT6600.020.1424121
GGGGATC30150.020.1005342
GTATGGC16350.019.925722
TCGCGGC24100.019.74323711
GGGATCC30350.019.7306733
Produced by FastQC (version 0.11.5)