FastQCFastQC Report
Fri 7 Aug 2020
HF2CNAFX2_n02_N2N3N1KO_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2CNAFX2_n02_N2N3N1KO_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8933170
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG496340.5556146362377521No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG173220.19390653038059277No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT123600.1383607386851476No Hit
CCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCA116920.13088299002481762No Hit
TGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACC112580.12602469224250742No Hit
TGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAA101270.11336401299874513No Hit
CCTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCC99140.11097964104567583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTA3000.024.50275437
CGTAGGG18450.024.47827364
TATCGCG9850.023.4418079
GTTAGTT31950.021.3787561
AACGTAG21950.021.05303462
GAACGTA22500.020.53817461
GTAGGGT22750.020.159565
CGACCCG18700.020.0149756
CGTGGCG18650.019.9088271
CGCTCGA6350.019.83141918
TCGCGAA3900.019.74061233
ATCGCGA3950.019.49018732
GGTAGTC23100.018.94587369
TAACGGC15200.018.8880469
TAGGGTA24100.018.59484366
GGGTAGT24000.018.52696268
ATCGCGG13050.018.4978810
TACCCTA37500.018.4721439
ACGCGAT4000.018.38345158
GGTAATC35700.018.13929437
Produced by FastQC (version 0.11.5)