FastQCFastQC Report
Fri 7 Aug 2020
HF2CNAFX2_n02_N2N3N1KO_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2CNAFX2_n02_N2N3N1KO_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21378495
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1834150.8579415903692005No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG323510.15132496464320805No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA261960.12253435052373893No Hit
TGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACC246460.11528407401924223No Hit
CCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCA241040.1127488160415408No Hit
GCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACGA225870.10565290026262372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGG13800.023.59510469
TCGTATA23550.023.47791331
TATCGCG37250.023.0086369
CTCGTAT24700.022.66567230
GTTAGTT85350.022.4925211
TACGTTC25450.022.26795610
CTAACGG15100.021.56343568
CGTATAA26450.021.1696232
ACTAACG15900.020.25766667
TACCCTA99750.020.1653619
ATATCGC44850.019.4219158
GGTAATC95450.019.18025237
ATCGCGG46200.018.62714210
ACCCTAC105250.018.34721210
GTACGAG101200.018.23334751
GGTAAGT31000.018.06902165
TCGCGGC49850.017.96502911
TAACGGC44650.017.6432769
ATTCGCG11900.017.34301618
GAACCGC106550.017.2850160
Produced by FastQC (version 0.11.5)