FastQCFastQC Report
Fri 7 Aug 2020
HF2CNAFX2_n02_N2N3N1KO_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2CNAFX2_n02_N2N3N1KO_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4409974
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG118860.2695253985624405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT6000.026.8245230
TCGTATA6200.025.39632231
CGTATAA6650.024.73236532
TATCGCG8000.024.0515969
TACGTTC6550.024.03490410
GGGGATC16650.023.7702162
ATTCGCG2300.022.81263718
GCTCGTA7150.022.50983229
GGTAAGT7500.021.9393265
CGCTAAT4800.021.13912824
TAACGGG3000.021.0071669
GTCCGTT4850.020.9221531
ATATCGC9400.020.8416168
CTAACGG3050.020.6627868
ATCGCGG9550.020.14793210
ATGTCCG5750.020.0801329
GGGGGAT19150.019.75551
ACTAACG3200.019.69421467
GGGATCC20450.018.8338933
TCGCGGC10500.018.65820511
Produced by FastQC (version 0.11.5)