FastQCFastQC Report
Fri 7 Aug 2020
HF2CNAFX2_n02_N2N3KO_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2CNAFX2_n02_N2N3KO_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4383050
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG563691.2860679207401238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGT5200.021.541914
GCGTTCG1200.001344141417.49561127
CGCGTGC6900.016.7301586
GCGCGTG7000.016.4930385
TGGCGCG7050.015.8963293
GTGGCGC8200.013.6711822
TAACGGC2751.4048521E-512.73105669
CGTAGGG5800.012.67545664
CCGGTAT2801.693035E-512.50200345
TCGCAAT4204.6766218E-912.501574540
CTTGACG4802.0190782E-1012.4095781
CGCACTA12750.012.35703759
TCGGCAT13050.012.07255369
GGTGGCG9950.011.9730611
AGTTCGG13550.011.88531566
ACGACCA11500.011.8768913
GTTCGGC13900.011.83791567
GACGACC12450.011.818162
GTCCGCA13150.011.71557756
CCGATCG13550.011.62534546
Produced by FastQC (version 0.11.5)