FastQCFastQC Report
Fri 7 Aug 2020
HF2CNAFX2_n02_N2N3KO_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2CNAFX2_n02_N2N3KO_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2067417
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG240381.1627068946419614No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG57310.27720580802034617No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT30400.1470433879570498No Hit
CGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAA22100.10689667348193423No Hit
CCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACGAGACC21450.10375265367364203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCCG3500.027.98456
TATCGCG3400.026.753779
GTTAGTT7050.025.3468251
GTGGCGA4200.024.1883932
CTTGACG1751.9826984E-1024.0262341
CGTGGCG4100.023.9285661
GGCGACC4250.023.8841534
TACCCTA7950.022.883739
GCGACCC4300.022.7786335
GGTAATC7550.022.71859437
GTACGAG7950.022.46104651
AACCGCA7950.022.45724161
GAACCGC8300.021.93467360
TGGCGAC4500.021.7904953
CGTAGGG4900.021.43433664
ATCGCGG4100.021.33326110
CGACGTA1656.837581E-821.20294422
ATATCGC4150.021.0757268
GGCACGC1003.9453217E-420.9919287
ACCCTAC8400.020.82532710
Produced by FastQC (version 0.11.5)