FastQCFastQC Report
Fri 7 Aug 2020
HF2CNAFX2_n02_N2N3KO_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2CNAFX2_n02_N2N3KO_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4730324
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1157672.4473376453705917No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG85590.1809389800783202No Hit
GCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACGA61710.13045618016863117No Hit
CCTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCC58900.12451578369684613No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGG48480.102487694289017No Hit
GGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCT48340.10219173147547608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG3000.031.48494518
ACTAACG3650.027.81668967
CGCGATT3700.027.42047921
TAACGGG3600.027.2322469
TCGCGAT3600.027.2103620
CTAACGG3650.026.85891268
TATCGCG11550.026.3549359
GGGGATC20750.023.803222
ATCGCGG13100.023.23660510
TAACGGT2650.022.44921932
CGTATAC2650.022.445429
ATATCGC13700.022.218948
TTCGCGA4500.021.76828819
TCGCGGC14550.021.40186911
CATTCGC4950.021.20198217
TACGTTC6650.020.5195410
GTTAGTT14300.020.336581
TCGCGAG2252.1282176E-1020.21554822
CCGTTAG2453.092282E-1119.9933998
CTCGTAT6850.019.41047130
Produced by FastQC (version 0.11.5)