FastQCFastQC Report
Fri 7 Aug 2020
HF2CNAFX2_n02_N2N3KO_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2CNAFX2_n02_N2N3KO_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4037041
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59478614.733216729778073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGT4400.019.8867054
CGCGTGC4850.018.0323836
TGGCGCG5300.016.5195453
CGACGCG2001.0021318E-515.7424655
CTAGCCT6750.015.5685592
GCGCGTG5900.014.8234145
TAGCCTT8350.013.8408073
CGGCTAG1800.001256300813.61674655
GTCGCGT1550.007394145713.54408730
CGCACTA13200.012.99731959
GTGGCGC6750.012.9737992
CTTGACG3002.4152414E-612.84565451
TCGGCAT12300.012.52566369
TGCGACA3651.2875353E-712.4596366
TTAGCTC9000.012.43741214
CCCGTTC8450.012.42840647
CGCGACG2559.7691125E-512.3605073
GTCCGCA14000.012.25507156
CTATTAG8300.012.22367111
ATCGGGT13850.012.13393249
Produced by FastQC (version 0.11.5)