FastQCFastQC Report
Fri 7 Aug 2020
HF2CNAFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2CNAFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9019840
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG789850.8756807216092524No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG384100.4258390392734239No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA214630.23795322311703976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC31450.043.95854613
ACGTCTG31050.043.18295715
CGTCTGA32950.040.6974116
CACACGT34750.040.18560412
CACGTCT34850.039.07122814
GCACACG37150.037.1174211
CCGATCG8250.034.3660270
GTCGGTT10250.032.469541
CCCGTAT5450.031.4974941
GATCGGA48250.029.8415161
AGAGCAC46500.029.8020748
TCGGAAG48600.029.6756383
CCAGTCA43800.029.48883826
GCCCTCT83400.029.4881441
ATCGGAA49650.029.2600572
CGGAAGA50050.029.162544
CAGTCAC43450.028.59394827
GAACTCC49600.027.88419321
AGCACAC50450.027.74848210
ACCCGTC4000.027.12222132
Produced by FastQC (version 0.11.5)