FastQCFastQC Report
Fri 7 Aug 2020
HF2CNAFX2_n01_RPE-1_WT_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2CNAFX2_n01_RPE-1_WT_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13685615
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA382450.27945401065279124No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG282700.20656726058712016No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG269090.19662251203179396No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT206690.1510271916899606No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT180830.13213143874060462No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC165270.12076183642459619No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT164190.11997268664944907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG21500.046.2350870
TATGCCG15750.046.22102748
GTCGGTT22750.041.5569761
TCGTATG18600.040.07961345
GTCCGGT20100.039.719191
CGTGCCG72600.036.49648369
TCCGTAT27800.035.75460437
GTTCCGT28300.035.12443535
ACTATCG77300.034.7284558
AGTTCCG29150.034.58051334
CCGTATC29000.033.5509838
TATCGGT81100.033.144560
TCGTGCC80650.032.9838268
ACGGTAC82500.032.79309545
ATCGGTC84100.032.00379661
GCACCGT22800.031.6369041
CCTTACG86650.031.34379641
GCCCTCT124250.031.1406351
TACGGTA87250.031.1281444
CTATCGG87350.030.73290459