FastQCFastQC Report
Fri 7 Aug 2020
HF2CNAFX2_n01_RPE-1_WT_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2CNAFX2_n01_RPE-1_WT_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8333050
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA521180.6254372648670055No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT404730.48569251354546056TruSeq Adapter, Index 13 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG280620.3367554496852893No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG267920.32151493150767124No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT180290.2163553560821068No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT156810.1881783980655342No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG136600.16392557346949796No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG100320.12038809319516865No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT95280.1143398875561769No Hit
GGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATT86370.10364752401581653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG46850.062.97616248
TCGTATG48100.062.5037845
CGTATGC51300.058.26378346
AATCTCG50800.057.04594841
ATGCCGT53100.055.03641549
ACACGTC61600.052.49912313
TGCCGTC55550.052.23103350
ATCTCGT55550.052.0420242
ACGTCTG62650.051.11646315
GTATGCC58550.051.0492247
GCCGTCT54600.050.31936651
CTCGTAT57550.050.11180544
TCTCGTA57650.050.0855943
CACACGT66450.048.7200312
CCGATCG19300.048.60313870
AGTCAAC66750.047.0867734
GTCCGGT16250.046.7553561
GCACACG69550.046.39750311
CACGTCT71100.045.13989314
CGTCTGA70750.045.06638316