Basic Statistics
Measure | Value |
---|---|
Filename | HF2CNAFX2_n01_RPE-1_WT_03.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 8333050 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA | 52118 | 0.6254372648670055 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT | 40473 | 0.48569251354546056 | TruSeq Adapter, Index 13 (97% over 40bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 28062 | 0.3367554496852893 | No Hit |
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 26792 | 0.32151493150767124 | No Hit |
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT | 18029 | 0.2163553560821068 | No Hit |
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT | 15681 | 0.1881783980655342 | No Hit |
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG | 13660 | 0.16392557346949796 | No Hit |
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG | 10032 | 0.12038809319516865 | No Hit |
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT | 9528 | 0.1143398875561769 | No Hit |
GGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATT | 8637 | 0.10364752401581653 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 4685 | 0.0 | 62.976162 | 48 |
TCGTATG | 4810 | 0.0 | 62.50378 | 45 |
CGTATGC | 5130 | 0.0 | 58.263783 | 46 |
AATCTCG | 5080 | 0.0 | 57.045948 | 41 |
ATGCCGT | 5310 | 0.0 | 55.036415 | 49 |
ACACGTC | 6160 | 0.0 | 52.499123 | 13 |
TGCCGTC | 5555 | 0.0 | 52.231033 | 50 |
ATCTCGT | 5555 | 0.0 | 52.04202 | 42 |
ACGTCTG | 6265 | 0.0 | 51.116463 | 15 |
GTATGCC | 5855 | 0.0 | 51.04922 | 47 |
GCCGTCT | 5460 | 0.0 | 50.319366 | 51 |
CTCGTAT | 5755 | 0.0 | 50.111805 | 44 |
TCTCGTA | 5765 | 0.0 | 50.08559 | 43 |
CACACGT | 6645 | 0.0 | 48.72003 | 12 |
CCGATCG | 1930 | 0.0 | 48.603138 | 70 |
AGTCAAC | 6675 | 0.0 | 47.08677 | 34 |
GTCCGGT | 1625 | 0.0 | 46.755356 | 1 |
GCACACG | 6955 | 0.0 | 46.397503 | 11 |
CACGTCT | 7110 | 0.0 | 45.139893 | 14 |
CGTCTGA | 7075 | 0.0 | 45.066383 | 16 |