HF2CNAFX2_n01_RPE-1_WT_02.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HF2CNAFX2_n01_RPE-1_WT_02.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6401830
Sequences flagged as poor quality	0
Sequence length	76
%GC	44

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA	12856	0.20081757872358375	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	9609	0.15009770643706566	No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	9053	0.14141268980900773	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC	8846	0.13817923937374157	TruSeq Adapter, Index 12 (100% over 50bp)
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT	8597	0.13428972653131996	No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT	7645	0.11941897863579634	No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT	6547	0.10226763284873232	No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC	6457	0.1008617848333992	No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG	6434	0.10050251256281408	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	1690	59.435936	46
TCGTATG	1725	57.012638	43
CGTATGC	2065	49.659397	44
GTCGTAT	860	48.428654	42
CCGATCG	1135	46.259056	70
GTCCGGT	1155	44.268295	1
ATGCCGT	2155	44.174892	47
ACACGTC	2915	43.944366	13
ACGTCTG	2950	43.067062	15
GTATGCC	2455	42.19822	45
GTCGGTT	1235	40.833584	1
CGTCTGA	3240	39.2123	16
CACACGT	3320	39.005363	12
TGCCGTC	2220	38.309563	48
AATCTCG	1650	38.180573	39
GCACACG	3510	36.794247	11
TCCGGTC	1030	36.359745	2
CGTGCCG	2735	36.34648	69
ACTATCG	2785	36.319523	58
CACGTCT	3520	36.19257	14