HF2CNAFX2_n01_RPE-1_WT_01.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HF2CNAFX2_n01_RPE-1_WT_01.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7510868
Sequences flagged as poor quality	0
Sequence length	76
%GC	43

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	30924	0.4117233853663784	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	28562	0.38027562193876924	No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC	11199	0.14910393845291914	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC	10577	0.1408226053233794	TruSeq Adapter, Index 7 (100% over 50bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	9520	0.1267496646193223	No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA	8776	0.11684401856083744	No Hit
GGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATT	7800	0.10384951512927666	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	1555	57.6188	46
ACACGTC	4790	52.607983	13
ACGTCTG	4795	52.261856	15
ATCGCGT	475	50.840538	40
GCACACG	5130	49.121292	11
CACACGT	5165	48.92395	12
TCGTATG	1675	48.89402	43
CGTATGC	1945	44.985764	44
CGTCTGA	5605	44.646854	16
CGCGTAT	270	44.07272	42
CACGTCT	5750	43.885605	14
GATCGGA	5770	43.205696	1
TCGGAAG	5830	42.86574	3
CGGAAGA	5860	42.82377	4
ATCGGAA	5845	42.81619	2
AGTCACC	5850	42.59698	28
ATGCCGT	1860	41.961075	47
ATCGCGG	320	41.561222	40
GTCGGTT	1235	41.392605	1
CATCGCG	925	40.48524	39