HF2CNAFX2_n01_N2N3N1KO_04.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HF2CNAFX2_n01_N2N3N1KO_04.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5273265
Sequences flagged as poor quality	0
Sequence length	76
%GC	45

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC	18896	0.3583358697125974	TruSeq Adapter, Index 6 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	16666	0.316047078991858	No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	16059	0.3045361839391724	No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC	12216	0.23165913338320754	No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT	10153	0.19253726107070288	No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG	8292	0.15724603258133243	No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCA	6314	0.11973606484786939	No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT	5950	0.1128333205329146	No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA	5372	0.10187236939543148	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	2100	61.16497	46
TCGTATG	2210	58.754025	43
CGCCAAT	2485	56.762215	33
CGTATGC	2325	55.54684	44
ACGCCAA	2530	55.47592	32
TATCTCG	2595	52.06021	39
AGTCACG	2795	50.588936	28
ATGCCGT	2625	48.531986	47
TGCCGTC	2655	47.983604	48
GTATGCC	2750	46.96233	45
GTCACGC	3000	46.66803	29
ATCTCGT	2930	46.585735	40
CGTCTGA	3130	45.50992	16
TCTCGTA	2990	44.363316	41
ACACGTC	3275	43.60184	13
ACGTCTG	3275	43.28124	15
GCCGTCT	2730	42.947525	49
CACACGT	3475	40.89095	12
CACGCCA	3490	40.31636	31
CGATCGG	645	39.072388	70