FastQCFastQC Report
Fri 7 Aug 2020
HF2CNAFX2_n01_N2N3N1KO_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2CNAFX2_n01_N2N3N1KO_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8933170
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA586100.6560940853023058No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT383960.4298138286856737No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT308300.34511825029636733No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG299770.33556956824956874No Hit
GGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGG233710.26162045500085634No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC213240.23870585693544394No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA203020.22726534925451994No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT192830.2158584242771603No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC191930.21485094317022962No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC170240.19057064849320007No Hit
GGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATT168100.18817508230560934No Hit
CTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT147940.16560750551036196No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT144310.16154399837907485No Hit
CCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAA140650.15744690854422339No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA140080.15680883717650063No Hit
GTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAA138810.1553871693922762No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG137520.15394311313900888No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC135820.15204009327036203TruSeq Adapter, Index 5 (98% over 50bp)
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG128430.14376755395900895No Hit
CCGGCTTCTATTGACTTGGGTTAATCGTGTGACCGCGGTGGCTGGCACGA124950.13987196034554364No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG121280.13576367627617072No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG117350.13136434210924006No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC116190.1300658109047516No Hit
CTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTCGGC112260.12566647673782097No Hit
TCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTG105430.11802081455966919No Hit
CTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCA102550.11479687501749099No Hit
GCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGA100710.1127371358655438No Hit
GTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGG99500.11138263348844811No Hit
GCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAG98810.11061023130646791No Hit
CTCGCCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACAT98480.11024082156726No Hit
CGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCA98010.10971469254475175No Hit
CGCCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGG92890.10398324446976828No Hit
GGCTAGGCTAAGCGTTTTGAGCTGCATTGCTGCGTGCTTGATGCTTGTCC92430.1034683096817815No Hit
GGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAG90870.10172200909643496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG29350.062.12796446
TCGTATG25100.058.4248843
CGTATGC33400.054.9088544
GTCGTAT20850.054.3871742
GTATGCC39750.047.10577845
ATGCCGT36100.045.5667647
TGCCGTC33050.044.90057848
GTCACAC79500.042.88186629
TGTCGTA27150.042.15367541
GCCGTCT29300.040.6133549
GATGTCG33500.040.43185839
GGCGTAT12500.039.75903742
ACGTCTG87000.039.22340415
CGTCTGA88100.038.77339616
CAGTCAC90450.037.92058627
GCGTGAA16650.037.83798259
CCAGTCA91800.037.4391826
ACACGTC91850.037.380913
CCGATCG10400.036.68467770
ACCCGTT43400.035.9678762
Produced by FastQC (version 0.11.5)