HF2CNAFX2_n01_N2N3N1KO_01.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HF2CNAFX2_n01_N2N3N1KO_01.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4409974
Sequences flagged as poor quality	0
Sequence length	76
%GC	44

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA	16203	0.367417132164498	No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC	11574	0.26245052691920634	No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT	9103	0.2064184505396177	No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT	8030	0.1820872413306745	No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT	6907	0.15662223858916174	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC	5453	0.12365152266203838	TruSeq Adapter, Index 2 (100% over 50bp)
GGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGG	5389	0.12220026694034931	No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC	5014	0.11369681544607746	No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG	5005	0.11349273261021493	No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT	4937	0.1119507734059203	No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG	4503	0.10210944554321635	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	785	54.393517	46
ACCGATG	1395	53.443127	32
TCGTATG	770	49.99872	43
TCACCGA	1695	44.810158	30
GTCACCG	1690	43.70008	29
CACCGAT	1740	43.047802	31
CGATGTA	1790	41.649815	34
CCGATGT	1835	40.819168	33
CGTATGC	1020	40.48916	44
ATCGCGT	150	39.66565	40
TATCTCG	1260	38.610123	39
GTATGCC	1260	35.832417	45
CCGATCG	875	35.202694	70
GTCGGTT	585	34.715317	1
ATGCCGT	1140	34.692093	47
GATGTAT	2165	34.59731	35
ACGTCTG	2305	33.404785	15
ATGTATC	2225	33.19016	36
CTAGTAC	1080	33.05471	9
ATCTCGT	1455	32.71394	40