FastQCFastQC Report
Fri 7 Aug 2020
HF2CNAFX2_n01_N2N3KO_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2CNAFX2_n01_N2N3KO_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4383050
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG168910.3853709175117783No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG145280.33145868744367507No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA125570.286490001254834No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC75630.17255107744607065No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG72390.165158964647905No Hit
GGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATT62990.14371271146804168No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT61210.139651612461642No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49270.11241030789062412No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA47860.1091933699136446No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT47550.10848609986196826No Hit
CGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCA47400.10814387241760874No Hit
GCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAG46450.10597643193666512No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT44800.10221193004871036TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7450.053.0858848
TCGTATG7950.049.74714745
CCGATCG4950.043.84025270
GTCGGTT7550.043.598411
CGTATGC9200.042.9880246
GTCCGGT5400.041.5026631
AACGATC9150.039.780438
AGTCACG10300.038.39706828
GAAACGA10300.037.37767836
TGAAACG10600.036.98186535
ATGCCGT10800.036.94349349
AAACGAT10400.036.3452237
TGGGGGG54350.035.7588231
ACGATCT10300.035.33889439
CGTGAAA11250.035.15625433
CGTGCGA708.709103E-734.999138
GCCGTCT11650.034.24804751
CGTGCCG27000.034.09404869
TGCCGTC11750.033.95657350
CACGTGA12000.032.9589931
Produced by FastQC (version 0.11.5)