FastQCFastQC Report
Fri 7 Aug 2020
HF2CNAFX2_n01_N2N3KO_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2CNAFX2_n01_N2N3KO_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4730324
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA607651.2845843117723015No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG502151.0615551915682733No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG304990.6447549892988302No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT286020.6046520280640396TruSeq Adapter, Index 16 (97% over 40bp)
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT223340.47214524840158945No Hit
GGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGG184880.3908400354817133No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT126190.2667681960051785No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC119160.25190663472523234No Hit
GTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAA115620.24442300358284128No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT102430.21653907850709592No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT92270.19506063432441414No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC87750.1855052634872368No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT83810.17717602430615745No Hit
GGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAG81440.17216579667692955No Hit
GCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGA74730.157980721827934No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT71410.15096217510682144No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA71260.15064507209231334No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC65470.1384048957323008No Hit
GCCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGA60220.1273062902245174No Hit
CCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAA57010.12052028571404412No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC53630.11337489778712832No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT50390.10652547267375341No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG50010.10572214503699957No Hit
CTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTCGGC49150.10390408775381983No Hit
CGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCA48770.10310076011706597No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG48280.10206489026967286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG34800.066.1763948
TCGTATG35800.064.23012545
ACCCGTC37900.062.0603232
CGTATGC37500.061.87834546
GTCCCGA38300.060.31170336
CGTCCCG39150.059.81062335
TCCCGAT38200.058.72879837
ATGCCGT39350.058.34659649
GTATGCC39900.058.3317747
TGCCGTC40250.056.69413850
GCCGTCT39900.055.5248351
CCGATCT38250.053.89398639
CTCGTAT43350.049.65265744
ACGTCTG48250.049.61525715
TCACCCG47600.049.4871130
GATCTCG42700.048.6872341
CCGTCCC48500.048.64095734
ACACGTC49550.048.59607713
CGTCTGA49350.048.50934216
CGATCTC42900.048.4602440
Produced by FastQC (version 0.11.5)