FastQCFastQC Report
Fri 7 Aug 2020
HF2CNAFX2_n01_N2N3KO_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2CNAFX2_n01_N2N3KO_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4037041
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2186005.414857069819207No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1783504.417839699918827No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG798931.9789989747441257No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA152210.37703357483860084No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG148090.36682808026968267No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA74200.18379798471207007No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT73950.18317871926492696No Hit
GGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATT68650.17005029178549338No Hit
GCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAG65410.1620246115905189No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG54540.1350989499487372No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG54190.1342319783227369No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT50950.12620629812776238No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG46730.11575309737998697No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG45080.11166594542884256No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTA40790.10103935035586707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG388100.055.305591
AGGGGGG206100.052.8875351
CCGATCG6300.050.0038670
TACTAAC24650.038.7656770
GTCCGGT3750.038.2828981
GTCGGTT5200.037.7082981
GCCCTCT39550.034.0852471
CCCGATC10050.032.73884269
CGTGCCG36100.030.73644369
TATACTA31450.030.60649768
ACCCGAT10550.030.52368268
ACTATCG37650.030.11960658
TATCGGT38550.029.32563460
GTGCCGG38100.029.1229870
CAATTGG18750.028.7456824
ACGGTAC39750.028.6153745
TCGTGCC39050.028.59375468
ATCGGTC39350.028.55154261
GACTATC40550.028.13713357
CCAATTG20550.027.9316063
Produced by FastQC (version 0.11.5)