FastQCFastQC Report
Wed 29 Jul 2020
HCY5TAFX2_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY5TAFX2_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5975819
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65579710.974177765424288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATA3750.033.71468431
TACGTTC3800.032.72718410
TATCGCG3100.032.69481322
CTCGTAT4100.030.835830
GCTCGTA4900.025.79090329
CGACGTA1659.276846E-1125.54275335
CGTATAA4850.025.34262532
ATATCGC4550.023.02073121
CTACGTT5450.022.7834919
TCCTACG5750.022.1510017
ATCGCGG4600.022.04484223
CGTTCCA5850.021.283312
CCTACGT6400.021.0073038
AGCTCGT6250.020.20539928
GGGATCC14250.019.6848553
GGGGATC16550.018.1536032
TCGACGT2357.605195E-917.93706334
GGATCCT15000.017.1530484
CGCCGTA1854.4724657E-617.13745147
CGTTCTA3307.2759576E-1217.03115334
Produced by FastQC (version 0.11.5)