FastQCFastQC Report
Wed 29 Jul 2020
HCY5TAFX2_n02_WT_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY5TAFX2_n02_WT_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82025354
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5450740.664518924234085No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG1332660.162469277487056No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA1239470.1511081561440137No Hit
GGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT1207490.14720936163225826No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT1035510.12624267369818362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG77500.043.3043222
TCGTATA106650.034.098631
CTCGTAT108850.033.44157830
CGTATAA112250.032.49101332
ATTCGCG49600.031.3248218
CGCTAAT94200.031.27987124
ATATCGC110600.031.102821
TACGTTC116250.031.06229410
GTCCGTT100600.028.87765531
CGCGATT55200.028.78179421
CCGCTAA103350.028.74846823
GCTCGTA134100.027.35142329
GGTAAGT138050.026.42289565
TTCGCGA60200.026.39044419
ATCGCGG132500.025.67248323
CTCGACG51950.025.06335633
CGACGTA53050.024.2793735
CGTTCTA121600.024.26475134
TCGCGAT67750.023.34692620
GGTGGCG561250.023.1794621
Produced by FastQC (version 0.11.5)