FastQCFastQC Report
Wed 29 Jul 2020
HCY5TAFX2_n02_WT_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY5TAFX2_n02_WT_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16268216
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG746970.4591591358265713No Hit
CCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCA283330.17416169050128175No Hit
GCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACGA237690.14610698554776994No Hit
TGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACC201920.12411932568389798No Hit
GGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTC190040.11681674253648955No Hit
GTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTC185450.11399528995680902No Hit
GCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAAT175300.10775613011285319No Hit
GGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGT171370.10534037659691757No Hit
GGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGC170600.10486706102254852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG18650.047.6616522
ATATCGC23550.038.3377821
CGACGTA11150.035.15842435
ATCGCGG26750.033.6224123
TCGACGT11800.032.92531234
CTCGACG11900.032.354533
CGCTCGA12200.032.13269431
TCGCGGC28200.031.7683324
ATTCGCG9250.028.7505418
CGCTAAT17450.026.67225324
CTCGTAT20850.025.85256230
TTCGCGA10700.025.83553519
TCGTATA21100.025.7121431
CGCGATT10350.025.35823821
CCGCTCG16000.024.93870230
GTCCGTT19100.024.7394131
CGTATAA22100.024.23193732
GATATCG37850.024.03840420
GACGTAG16650.023.75475136
TCGCGAT11200.023.74617820
Produced by FastQC (version 0.11.5)