FastQCFastQC Report
Wed 29 Jul 2020
HCY5TAFX2_n02_WT_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY5TAFX2_n02_WT_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18047775
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG595210.3297968863197818No Hit
GGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT204770.11345996944221656No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA204720.11343226519612529No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG22650.057.47722622
ATATCGC29600.044.3350721
CGACGTA14500.036.93133535
ATCGCGG36950.035.2331923
CTCGACG15950.033.57394433
TCGACGT17400.031.98302334
CGCTCGA17900.029.91644531
TCGCGGC45150.029.22051224
CGCGATT10100.027.37244221
GATATCG49050.027.32546820
ATTCGCG9750.027.2773618
GACGTAG21000.025.83354836
CCGCTCG20850.025.68366430
TCGCGAT11200.024.99653420
CTCGTAT24100.024.83471140
TTCGCGA11350.024.66536519
TCGTATA24150.024.63836141
CGTATAA25100.023.8448242
GTATGGC57900.022.9871372
CGCTAAT18650.022.7044424
Produced by FastQC (version 0.11.5)