FastQCFastQC Report
Wed 29 Jul 2020
HCY5TAFX2_n02_WT_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY5TAFX2_n02_WT_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11184148
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG377280.3373345917811531No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG7900.045.62627822
TCGTATA16750.038.03061331
CGTATAA16950.037.7883732
TACGTTC16900.037.47405610
CTCGTAT17650.036.2896830
CGCTAAT13000.034.18596324
ATTCGCG5550.032.78597318
GTCCGTT13550.032.03016331
GCTCGTA20500.031.58331729
GGGGATC52000.030.3789312
ATATCGC12200.029.83092721
TTCGCGA6350.029.20652219
ACGTTCC22250.028.9357311
GGTAAGT22950.028.52483265
TCCTACG25900.027.289797
CGCGATT6550.026.71295721
ATCGCGG13900.025.93171323
CGTTCTA17500.025.80051634
ACCGCTA17650.025.57701722
TCGCGAT7150.025.45016120
Produced by FastQC (version 0.11.5)