FastQCFastQC Report
Wed 29 Jul 2020
HCY5TAFX2_n02_N2_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY5TAFX2_n02_N2_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5714273
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG648891.1355600266210593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG6000.044.9089222
ATATCGC7500.036.3930921
ATCGCGG7550.035.688923
TCGCGGC8450.031.88743624
CTCGTAT6900.031.45073530
TCGTATA7150.030.84059331
CTCGACG3450.030.43619533
CGTATAA7650.029.28240432
TACGTTC7350.029.03955810
CGACGTA4050.026.790935
CGCTCGA4000.026.25121731
GCTCGTA8650.025.08545329
AATCGCG700.001228385524.9956875
TCGACGT4450.024.38315434
ACGTTCC9100.023.8395411
GGTAAGT9700.022.01518465
ATTCGCG2600.021.53305218
AGCTCGT10600.021.12898628
GGGGATC20650.020.8601112
CCGCTCG5200.020.19324530
Produced by FastQC (version 0.11.5)