FastQCFastQC Report
Wed 29 Jul 2020
HCY5TAFX2_n02_N2_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY5TAFX2_n02_N2_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5512485
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG397720.721489491581383No Hit
GGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCCATCTC78020.14153326494312457No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC71100.12897994280256544No Hit
CGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAA68400.12408197029107562No Hit
TGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTTT61830.11216357051311704No Hit
GCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCCATCTCTC59080.10717489480697No Hit
CGGAACGGCGCTCGCCCATCTCTCAGGACCGACTGACCCATGTTCAACTG58120.10543339346955138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT6550.043.2827430
CGTATAA6650.042.63187832
TCGTATA6650.042.63187831
TACGTTC7000.038.99036810
ATTCGCG2800.036.2430218
CGCTAAT3950.034.5515624
TATCGCG2550.034.30867422
CGCGATT3000.032.66156421
TCCTACG8900.030.6676927
CTACGTT9650.030.4587979
GTCCGTT4500.029.55576531
GGTAAGT9500.029.47897365
CCGCTAA4700.029.03854223
TCGCGAG1601.8189894E-1228.43331322
GGGATCC22250.028.0075653
CGAGCTC10700.027.47259126
CGTTCCA10850.026.4450812
CCTACGT10650.026.2848248
GCTCGTA11500.024.95511229
CGTTCTA5500.024.81835634
Produced by FastQC (version 0.11.5)