FastQCFastQC Report
Wed 29 Jul 2020
HCY5TAFX2_n02_N2_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY5TAFX2_n02_N2_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9897750
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG419540.4238741128034149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATA16950.043.98383331
CTCGTAT17000.043.236830
TATCGCG7350.040.94656422
TACGTTC18450.040.39607210
CGTATAA18700.039.68052732
ATTCGCG5800.036.19937518
CCGCTAA9450.035.18054223
CGCGATT6100.034.99414421
TCCTACG22500.034.3702937
CTACGTT22050.034.118179
GGTAAGT22000.034.0507765
GCTCGTA22500.032.97559429
CGCTAAT10200.032.24983224
CCTACGT24000.031.6379838
GGGGATC51350.031.3751582
CGTTCCA24150.031.00632912
GTCCGTT10850.030.00104331
GGGATCC51350.030.000253
AGCTCGT25350.029.54304928
GGGGGAT54150.028.9428461
Produced by FastQC (version 0.11.5)