FastQCFastQC Report
Wed 29 Jul 2020
HCY5TAFX2_n02_N2_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY5TAFX2_n02_N2_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14433464
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1931191.33799481538181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG13400.049.88242322
ATTCGCG7450.038.04662318
CTCGTAT19700.036.2453430
ATCGCGG18550.036.22270223
ATATCGC18800.035.55252521
CGCTAAT17500.033.995124
CGCGATT8650.033.17360321
TCGTATA21550.033.1337931
TCGCGGC20850.032.05772824
GTCCGTT17850.031.7661831
CGACGTA8300.031.62720735
TACGTTC22800.031.30650510
CGTATAA23200.031.22989732
CTCGACG8750.031.20144833
TCGCGAT9000.031.1059820
GGGGATC62250.030.4378592
TTCGCGA9450.029.62412819
CGCTCGA9200.029.29483831
GCTCGTA25250.028.41450129
TCGACGT9950.027.08668534
Produced by FastQC (version 0.11.5)