FastQCFastQC Report
Wed 29 Jul 2020
HCY5TAFX2_n01_WT_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY5TAFX2_n01_WT_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82025354
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG6686610.8151881916900963No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT3931160.479261570757744No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT3553440.43321239430432695No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC3125230.3810078040016749No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2471460.3013043991251778No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG2425570.29570978748839044No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA2246250.27384825428488857No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC1736760.2117345327153358No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCA1722690.21001920942639274No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1636460.19950660621348856No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1537020.18738352534266417TruSeq Adapter, Index 6 (100% over 50bp)
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT1203880.1467692538090113No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT998510.12173187329371354No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA836630.10199651195653481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG201550.058.7786246
TCGTATG214050.054.33236743
CGCCAAT275550.050.53852533
ACGCCAA279350.049.21185332
TATCTCG267000.046.37588539
CGATCGG492000.045.8428170
ACGTCTG325900.042.56896215
CGTATGC281200.042.17933744
ACACGTC334600.041.6922313
CGTCTGA334100.041.3460916
ATGCCGT285150.040.31527747
AGTCACG351400.039.23106428
ATCTCGT319450.038.7503540
GTATGCC313650.038.51846745
CCGATCG1263650.038.162870
TCTCGTA314900.037.55424541
CACACGT375750.037.13564712
TGCCGTC311050.036.08055548
CCCGATC1373350.035.14802669
CACGTCT397100.035.14787314