Basic Statistics
Measure | Value |
---|---|
Filename | HCY5TAFX2_n01_WT_4.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 82025354 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 47 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG | 668661 | 0.8151881916900963 | No Hit |
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT | 393116 | 0.479261570757744 | No Hit |
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT | 355344 | 0.43321239430432695 | No Hit |
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC | 312523 | 0.3810078040016749 | No Hit |
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT | 247146 | 0.3013043991251778 | No Hit |
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG | 242557 | 0.29570978748839044 | No Hit |
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA | 224625 | 0.27384825428488857 | No Hit |
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC | 173676 | 0.2117345327153358 | No Hit |
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCA | 172269 | 0.21001920942639274 | No Hit |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 163646 | 0.19950660621348856 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC | 153702 | 0.18738352534266417 | TruSeq Adapter, Index 6 (100% over 50bp) |
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT | 120388 | 0.1467692538090113 | No Hit |
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT | 99851 | 0.12173187329371354 | No Hit |
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA | 83663 | 0.10199651195653481 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 20155 | 0.0 | 58.77862 | 46 |
TCGTATG | 21405 | 0.0 | 54.332367 | 43 |
CGCCAAT | 27555 | 0.0 | 50.538525 | 33 |
ACGCCAA | 27935 | 0.0 | 49.211853 | 32 |
TATCTCG | 26700 | 0.0 | 46.375885 | 39 |
CGATCGG | 49200 | 0.0 | 45.84281 | 70 |
ACGTCTG | 32590 | 0.0 | 42.568962 | 15 |
CGTATGC | 28120 | 0.0 | 42.179337 | 44 |
ACACGTC | 33460 | 0.0 | 41.69223 | 13 |
CGTCTGA | 33410 | 0.0 | 41.34609 | 16 |
ATGCCGT | 28515 | 0.0 | 40.315277 | 47 |
AGTCACG | 35140 | 0.0 | 39.231064 | 28 |
ATCTCGT | 31945 | 0.0 | 38.75035 | 40 |
GTATGCC | 31365 | 0.0 | 38.518467 | 45 |
CCGATCG | 126365 | 0.0 | 38.1628 | 70 |
TCTCGTA | 31490 | 0.0 | 37.554245 | 41 |
CACACGT | 37575 | 0.0 | 37.135647 | 12 |
TGCCGTC | 31105 | 0.0 | 36.080555 | 48 |
CCCGATC | 137335 | 0.0 | 35.148026 | 69 |
CACGTCT | 39710 | 0.0 | 35.147873 | 14 |