FastQCFastQC Report
Wed 29 Jul 2020
HCY5TAFX2_n01_WT_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY5TAFX2_n01_WT_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18047775
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG942780.522380182598686No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT736960.40833842398855263No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT588780.32623412027244353No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT484090.26822696980652744No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC449140.24886170178872466TruSeq Adapter, Index 4 (100% over 50bp)
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC412110.22834393713352477No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG407650.22587271838218284No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA330960.18337994572738192No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC266940.1479074290321106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG65800.060.0490746
TCGTATG67250.058.7022943
ACACGTC90000.049.22995813
CGTATGC81500.049.16840444
ACGTCTG89500.049.11394515
CGTCTGA92800.047.1411616
ATGCCGT83350.045.57013347
GATCGGA97400.045.0188261
GTATGCC91650.043.91406245
CACACGT101050.043.67339712
GACCAAT95200.042.60772335
CCGATCG160100.042.56558270
TGCCGTC89800.042.29748
AATCTCG86350.040.9759939
ATCGGAA108800.040.766542
CGGAAGA111400.040.2858924
CACGTCT109900.040.2838614
GCCGTCT86700.040.2157749
TCGGAAG110850.040.1383973
GCACACG112100.039.61815611