Basic Statistics
Measure | Value |
---|---|
Filename | HCY5TAFX2_n01_WT_2.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 18047775 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 47 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG | 94278 | 0.522380182598686 | No Hit |
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT | 73696 | 0.40833842398855263 | No Hit |
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT | 58878 | 0.32623412027244353 | No Hit |
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT | 48409 | 0.26822696980652744 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC | 44914 | 0.24886170178872466 | TruSeq Adapter, Index 4 (100% over 50bp) |
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC | 41211 | 0.22834393713352477 | No Hit |
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG | 40765 | 0.22587271838218284 | No Hit |
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA | 33096 | 0.18337994572738192 | No Hit |
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC | 26694 | 0.1479074290321106 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 6580 | 0.0 | 60.04907 | 46 |
TCGTATG | 6725 | 0.0 | 58.70229 | 43 |
ACACGTC | 9000 | 0.0 | 49.229958 | 13 |
CGTATGC | 8150 | 0.0 | 49.168404 | 44 |
ACGTCTG | 8950 | 0.0 | 49.113945 | 15 |
CGTCTGA | 9280 | 0.0 | 47.14116 | 16 |
ATGCCGT | 8335 | 0.0 | 45.570133 | 47 |
GATCGGA | 9740 | 0.0 | 45.018826 | 1 |
GTATGCC | 9165 | 0.0 | 43.914062 | 45 |
CACACGT | 10105 | 0.0 | 43.673397 | 12 |
GACCAAT | 9520 | 0.0 | 42.607723 | 35 |
CCGATCG | 16010 | 0.0 | 42.565582 | 70 |
TGCCGTC | 8980 | 0.0 | 42.297 | 48 |
AATCTCG | 8635 | 0.0 | 40.97599 | 39 |
ATCGGAA | 10880 | 0.0 | 40.76654 | 2 |
CGGAAGA | 11140 | 0.0 | 40.285892 | 4 |
CACGTCT | 10990 | 0.0 | 40.28386 | 14 |
GCCGTCT | 8670 | 0.0 | 40.21577 | 49 |
TCGGAAG | 11085 | 0.0 | 40.138397 | 3 |
GCACACG | 11210 | 0.0 | 39.618156 | 11 |