Basic Statistics
Measure | Value |
---|---|
Filename | HCY5TAFX2_n01_WT_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 11184148 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG | 29462 | 0.2634264138850809 | No Hit |
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA | 25333 | 0.22650808984287402 | No Hit |
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT | 21441 | 0.19170883647104814 | No Hit |
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC | 20496 | 0.18325937746889617 | No Hit |
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT | 17506 | 0.15652511036155814 | No Hit |
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT | 14792 | 0.132258621756436 | No Hit |
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC | 14559 | 0.13017531599188423 | No Hit |
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT | 13555 | 0.12119832462875134 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ATCGCGT | 1210 | 0.0 | 60.739178 | 40 |
ACCGATG | 6645 | 0.0 | 59.62005 | 32 |
TATGCCG | 2145 | 0.0 | 57.268368 | 46 |
CGCGTAT | 890 | 0.0 | 55.83845 | 42 |
GTCACCG | 7100 | 0.0 | 55.79982 | 29 |
TGTATCG | 3415 | 0.0 | 54.52001 | 37 |
CACCGAT | 7370 | 0.0 | 54.135006 | 31 |
TCACCGA | 7495 | 0.0 | 53.326023 | 30 |
GTATCGC | 2040 | 0.0 | 52.83912 | 38 |
CGATGTA | 7500 | 0.0 | 52.682896 | 34 |
ACACGTC | 7865 | 0.0 | 51.48369 | 13 |
CCGATGT | 7730 | 0.0 | 51.296917 | 33 |
ACGTCTG | 7890 | 0.0 | 50.78828 | 15 |
CGTCTGA | 8220 | 0.0 | 48.791912 | 16 |
CACACGT | 8450 | 0.0 | 47.71235 | 12 |
TCGCGTA | 1055 | 0.0 | 47.43715 | 41 |
GATGTAT | 8430 | 0.0 | 45.874535 | 35 |
TCGTATG | 1970 | 0.0 | 45.83408 | 43 |
GCACACG | 8915 | 0.0 | 45.26316 | 11 |
ATGTATC | 7955 | 0.0 | 45.225975 | 36 |