FastQCFastQC Report
Wed 29 Jul 2020
HCY5TAFX2_n01_WT_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY5TAFX2_n01_WT_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11184148
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG294620.2634264138850809No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA253330.22650808984287402No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT214410.19170883647104814No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC204960.18325937746889617No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT175060.15652511036155814No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT147920.132258621756436No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC145590.13017531599188423No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT135550.12119832462875134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGT12100.060.73917840
ACCGATG66450.059.6200532
TATGCCG21450.057.26836846
CGCGTAT8900.055.8384542
GTCACCG71000.055.7998229
TGTATCG34150.054.5200137
CACCGAT73700.054.13500631
TCACCGA74950.053.32602330
GTATCGC20400.052.8391238
CGATGTA75000.052.68289634
ACACGTC78650.051.4836913
CCGATGT77300.051.29691733
ACGTCTG78900.050.7882815
CGTCTGA82200.048.79191216
CACACGT84500.047.7123512
TCGCGTA10550.047.4371541
GATGTAT84300.045.87453535
TCGTATG19700.045.8340843
GCACACG89150.045.2631611
ATGTATC79550.045.22597536