Basic Statistics
Measure | Value |
---|---|
Filename | HCY5TAFX2_n01_N2_04.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 5714273 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA | 20336 | 0.35588079183476184 | No Hit |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 19072 | 0.3337607426176523 | No Hit |
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG | 11967 | 0.2094229659660993 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT | 10457 | 0.18299790717034345 | TruSeq Adapter, Index 14 (97% over 44bp) |
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG | 10213 | 0.1787278976695723 | No Hit |
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT | 9527 | 0.1667228709583879 | No Hit |
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT | 8443 | 0.14775282875004397 | No Hit |
GGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATT | 8374 | 0.1465453260633505 | No Hit |
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC | 7857 | 0.1374978059326182 | No Hit |
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 7732 | 0.13531030106541986 | No Hit |
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT | 6085 | 0.10648773693521468 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 1505 | 0.0 | 59.315483 | 48 |
ATCGCGT | 490 | 0.0 | 59.28227 | 42 |
CGCGTAT | 385 | 0.0 | 56.360363 | 44 |
GTTCCGT | 3360 | 0.0 | 55.93523 | 35 |
TCCGTAT | 3310 | 0.0 | 55.72232 | 37 |
TCGTATG | 1345 | 0.0 | 55.164078 | 45 |
AGTTCCG | 3495 | 0.0 | 54.275333 | 34 |
CCGTATC | 3235 | 0.0 | 53.551754 | 38 |
GTATCGC | 830 | 0.0 | 53.12944 | 40 |
CGTATCG | 1270 | 0.0 | 49.327843 | 39 |
CGTATCT | 2260 | 0.0 | 48.935207 | 39 |
TTCCGTA | 3860 | 0.0 | 48.599064 | 36 |
ACGTCTG | 3935 | 0.0 | 48.56177 | 15 |
CGTATGC | 1755 | 0.0 | 48.2593 | 46 |
ACACGTC | 4060 | 0.0 | 47.411453 | 13 |
TATCTCG | 1970 | 0.0 | 47.256138 | 41 |
CACACGT | 4260 | 0.0 | 44.774784 | 12 |
CGTCTGA | 4340 | 0.0 | 43.788162 | 16 |
TCGCGTA | 505 | 0.0 | 43.660828 | 43 |
CACGTCT | 4385 | 0.0 | 43.65805 | 14 |