FastQCFastQC Report
Wed 29 Jul 2020
HCY5TAFX2_n01_N2_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY5TAFX2_n01_N2_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5714273
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA203360.35588079183476184No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG190720.3337607426176523No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG119670.2094229659660993No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT104570.18299790717034345TruSeq Adapter, Index 14 (97% over 44bp)
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG102130.1787278976695723No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT95270.1667228709583879No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT84430.14775282875004397No Hit
GGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATT83740.1465453260633505No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC78570.1374978059326182No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77320.13531030106541986No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT60850.10648773693521468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG15050.059.31548348
ATCGCGT4900.059.2822742
CGCGTAT3850.056.36036344
GTTCCGT33600.055.9352335
TCCGTAT33100.055.7223237
TCGTATG13450.055.16407845
AGTTCCG34950.054.27533334
CCGTATC32350.053.55175438
GTATCGC8300.053.1294440
CGTATCG12700.049.32784339
CGTATCT22600.048.93520739
TTCCGTA38600.048.59906436
ACGTCTG39350.048.5617715
CGTATGC17550.048.259346
ACACGTC40600.047.41145313
TATCTCG19700.047.25613841
CACACGT42600.044.77478412
CGTCTGA43400.043.78816216
TCGCGTA5050.043.66082843
CACGTCT43850.043.6580514