FastQCFastQC Report
Wed 29 Jul 2020
HCY5TAFX2_n01_N2_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY5TAFX2_n01_N2_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9897750
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCA368070.37187239524134275No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC296540.2996034452274507No Hit
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT183940.18584021621075497No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCATG171450.17322118663332575No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT166910.16863428556995277No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA149820.15136773509130863No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG133060.13443459372079514No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG128250.12957490338713346No Hit
ATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTA127280.12859488267535552No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA113860.1150362456113763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG19000.056.91759546
TCGTATG21150.047.49120743
CGTATGC23400.047.2620944
ATCGCGG29050.046.86533734
TCGACGT14950.046.351745
TCGCGGC30200.045.08072735
TATCGCG30050.045.07282333
GTCGTAT12300.042.96487442
ATATCGC31450.042.9546832
CTCGACG17050.041.4637644
CGACGTA17500.039.79758546
GTCGGTT15900.039.645671
GCTCGAC18450.039.6452943
CGTCTGA49500.038.9572316
CTAGTAC35800.038.7129339
CGATCGG13700.037.5562670
ACGTCTG51600.037.50741615
ATGCCGT25150.036.74624347
ACACGTC54100.035.9035513
CGCGTAT2250.035.77560442