FastQCFastQC Report
Wed 29 Jul 2020
HCY5TAFX2_n01_N2_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY5TAFX2_n01_N2_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14433464
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG912250.6320381579917336No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA454810.31510800179360965No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG378590.2623001657814091No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT345740.23954055658433762No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC276640.1916657013174384No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT273370.18940013291334637No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG268920.18631701994753302No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT206810.14328507695727097No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG199520.1382343143683318No Hit
GGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGG185510.12852770478382736No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC178190.12345615716365801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGT9600.051.403340
TATGCCG14100.046.6639946
CGCGTAT6150.045.5258442
CATCGCG17350.043.16769839
CGTCTGA86600.042.6361216
ACGTCTG86600.042.55529415
ACACGTC87950.042.14084213
CACACGT94600.039.36348712
TATCGCG39950.038.37116233
GCACACG99800.037.59303311
CACGTCT99750.037.12067414
AGGGGGG136650.036.210121
ATATCGC43100.035.5666532
ATCGCGG43750.035.11810734
TCGACGT18350.035.09335345
ACCAGAT106350.034.98177732
GTCGGTT17100.034.4041331
CCGATCG42350.033.63804270
TCGCGGC46400.033.41414635
CTAGTAC47850.032.401489