FastQCFastQC Report
Fri 31 Jul 2020
HCY32AFX2_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY32AFX2_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10276998
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG120335111.709168377769462No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2626092.5553084665385746Illumina Single End PCR Primer 1 (100% over 50bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCCGATTAGATCGGAAGAGCGTCG401110.3902988012647273Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCCGATAGATCGGAAGAGCGTCGT288550.28077265364846815Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG262050.25498691349360975No Hit
GTGACTGGAGTTCAGACGTGTGCTCTTCCGAGATCGGAAGAGCGTCGTGT138870.13512700887944123Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG134680.1310499427945787No Hit
GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCAGATCGGAAGAGCGTCG124840.12147516229934073Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ATATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC123150.11983071321021956Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCCGAAGATCGGAAGAGCGTCGTG102780.10000974992891894Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTGA902500.067.6683042
ACTGAAG909600.067.115564
AGATCAT661400.066.946349
GATCATC419550.066.91710
GCACACG160700.066.650118
CTGAAGA973150.066.475765
GTGACTG250900.066.46351
ATAACTG923050.066.2890241
ATCATCC392150.066.0950111
TGACTGG256750.066.014642
CGTGTGC286450.065.9005917
ACGTGTG288150.065.8596116
TCAGACG291050.065.7737612
TGAAGAT1004300.065.627486
GTGTGCT283150.065.5993918
ACTGGAG267950.065.409034
AGTTCAG297150.065.268839
AGAGCAC194950.065.1796415
AACTGAA947400.065.068083
CAGACGT294900.064.9098313
Produced by FastQC (version 0.11.5)