FastQCFastQC Report
Fri 31 Jul 2020
HCY32AFX2_n02_ncm8-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY32AFX2_n02_ncm8-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2279
Sequences flagged as poor quality0
Sequence length76
%GC37

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC763.3347959631417288Illumina Single End PCR Primer 1 (100% over 50bp)
ATATCTGAAGATCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC381.6673979815708644Illumina Single End PCR Primer 1 (97% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG200.8775778850372972No Hit
ATATCTGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC190.8336989907854322Illumina Single End PCR Primer 1 (100% over 44bp)
ATAACTGAAGATCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC90.39491004826678366Illumina Single End PCR Primer 1 (100% over 38bp)
ATATCGGAAGATCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC70.307152259763054Illumina Single End PCR Primer 1 (100% over 38bp)
ATATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC60.26327336551118913Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAGTTCAGACGTGTGCTCAGATCGGAAGAGCGTCGTGTAGGGA30.13163668275559456Illumina Single End PCR Primer 1 (100% over 25bp)
AGATCTGAAGATCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC30.13163668275559456Illumina Single End PCR Primer 1 (100% over 38bp)
TGTCATGATTCAGAGCCGACAATCTGTAGAAAAGTTAATGAAATTCTTCA30.13163668275559456No Hit
ATATCTGAAGATCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC30.13163668275559456Illumina Single End PCR Primer 1 (96% over 31bp)
GTGACTGGAGTTCAGACGTGTGCTAGATCGGAAGAGCGTCGTGTAGGGAA30.13163668275559456Illumina Single End PCR Primer 1 (100% over 26bp)
CGTGCAGTGTAAACAACTTTTCAACAAGATCTTTGACTAAAAGTCAATGC30.13163668275559456No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAGAT206.3353546E-570.000016
TCGTCGT206.3353546E-570.0000112
ATAACTG150.001946575970.01
TAACTGA150.001946575970.02
TATCTGA150.001946575970.02
ATATCTG150.001946575970.01
ATCTGAA150.001946575970.03
GTCGCCG351.8479477E-760.045
GTATCAT351.8479477E-760.051
CCGTATC351.8479477E-760.049
TCGCCGT351.8479477E-760.046
CGGTGGT351.8479477E-760.040
CGTATCA351.8479477E-760.050
GCCGTAT351.8479477E-760.048
CGCCGTA351.8479477E-760.047
GGTCGCC351.8479477E-760.044
ATCTCGG305.979993E-658.33333236
TCGGTGG305.979993E-658.33333239
GAAGATC305.979993E-658.3333327
TCTCGGT305.979993E-658.33333237
Produced by FastQC (version 0.11.5)