FastQCFastQC Report
Fri 31 Jul 2020
HCY32AFX2_n02_ncm8-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCY32AFX2_n02_ncm8-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16090088
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC11417417.0959276294822Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2438391.5154609471371443No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC447040.2778356463929843Illumina Single End PCR Primer 1 (100% over 49bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC443540.2756603941507343Illumina Single End PCR Primer 1 (100% over 49bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC220500.13704089126175073Illumina Single End PCR Primer 1 (98% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC201550.1252634541215685Illumina Single End PCR Primer 1 (100% over 49bp)
AGTTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC166480.1034674266542234Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAG95300.054.0599638
GCACACG56300.047.74343518
GTGACTG80550.047.310661
GAGCACA115700.046.6154716
AGATCGA115350.045.391537
AGCACAC101250.045.179517
TGACTGG85600.044.6576732
CCGTATC2090900.044.65275649
AGAGCAC126850.044.6425415
GTATCAT2068300.044.4891851
CGTATCA2112350.044.3103450
CGCCGTA2137500.044.23926547
GCCGTAT2128700.044.0719448
AGATAGA200250.043.9043927
TCGCCGT2130500.043.8753646
GGTCGGA42050.043.7891962
GACTGGA90050.043.4917833
CATTAAA2176700.043.3525255
AAAGGGG1715750.043.34684467
GGTCGCC2183150.043.2917744
Produced by FastQC (version 0.11.5)