HCY32AFX2_n02_ncm8-7.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HCY32AFX2_n02_ncm8-7.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15535705
Sequences flagged as poor quality	0
Sequence length	76
%GC	41

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	1150420	7.405006724831606	Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	293676	1.8903294057141276	No Hit
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC	63548	0.4090448421877218	Illumina Single End PCR Primer 1 (100% over 49bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC	56277	0.36224297513373227	Illumina Single End PCR Primer 1 (100% over 49bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	30339	0.1952856339638272	Illumina Single End PCR Primer 1 (98% over 50bp)
AGTTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	23566	0.15168928606715948	Illumina Single End PCR Primer 1 (98% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC	21364	0.13751548449201373	Illumina Single End PCR Primer 1 (100% over 49bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GATCGAG	6840	48.610264	8
GGTCGGA	4975	48.41082	2
GCACACG	5055	45.211872	18
AAAGGGG	185860	44.683464	67
AAGGGGG	186460	44.587334	68
AGATAGA	20140	43.757927	7
CCGTATC	223190	43.66338	49
AGGTCGG	5445	43.55267	1
GATAGAT	19820	43.528503	8
GTATCAT	221745	43.523335	51
CGCCGTA	227225	43.51492	47
CGTATCA	225060	43.418774	50
AGGGGGG	194390	43.308567	69
TCGCCGT	226835	43.248745	46
GCCGTAT	226980	43.214947	48
GGTCGCC	230780	42.900715	44
CTCGGTG	225325	42.88775	38
GTCGCCG	231075	42.803535	45
TCTCGGT	225465	42.715195	37
GTGGTCG	231275	42.715065	42