HCY32AFX2_n02_ncm8-6.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HCY32AFX2_n02_ncm8-6.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14984213
Sequences flagged as poor quality	0
Sequence length	76
%GC	41

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	1109459	7.404185992284012	Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	233803	1.5603288607816774	No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC	62614	0.41786645718397086	Illumina Single End PCR Primer 1 (100% over 49bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC	60644	0.4047192868921444	Illumina Single End PCR Primer 1 (100% over 49bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	30561	0.2039546554764004	Illumina Single End PCR Primer 1 (98% over 50bp)
AGTTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	24761	0.16524725055630216	Illumina Single End PCR Primer 1 (98% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC	23243	0.15511658837204198	Illumina Single End PCR Primer 1 (100% over 49bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GATCGAG	7470	52.24147	8
GGTCGGA	5105	50.3298	2
AAAGGGG	174215	47.009377	67
AAGGGGG	175315	46.715168	68
AGGTCGG	5485	46.42551	1
GCACACG	5295	45.54224	18
AGTTCGG	4570	45.527016	1
AGGGGGG	181775	45.38808	69
AAAAGGG	183545	44.8734	66
AGATAGA	19980	44.388683	7
GATAGAT	20025	43.7646	8
CCGTATC	220700	42.714542	49
CGCCGTA	224245	42.644863	47
GTATCAT	218490	42.642006	51
GTTCGGA	4940	42.586548	2
CGTATCA	222480	42.462467	50
TCGCCGT	224210	42.368977	46
GCCGTAT	223910	42.353844	48
CGATCGG	1910	42.17872	1
GTCGCCG	227130	42.11552	45