HCY32AFX2_n02_ncm8-5.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HCY32AFX2_n02_ncm8-5.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13157608
Sequences flagged as poor quality	0
Sequence length	76
%GC	42

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	483376	3.6737376580910452	Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	243664	1.8518867563161936	No Hit
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	22677	0.17234895582844542	Illumina Single End PCR Primer 1 (98% over 50bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC	22107	0.16801686142344413	Illumina Single End PCR Primer 1 (100% over 49bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTCTCGGTGGTCGCC	21455	0.16306155343737253	Illumina Single End PCR Primer 1 (98% over 50bp)
AGTTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	19853	0.15088608810963208	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCC	16312	0.12397390163926451	Illumina Single End PCR Primer 1 (98% over 50bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC	16029	0.12182305476800952	Illumina Single End PCR Primer 1 (100% over 49bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTGGCC	15455	0.117460559700517	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	14876	0.11306006380491043	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGGGGTCGCC	13529	0.10282264071098637	Illumina Single End PCR Primer 1 (98% over 50bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CCGTATC	105430	52.709953	49
CGCCGTA	109200	52.23313	47
TCGCCGT	110000	51.57962	46
CGTATCA	110395	51.45847	50
ATCTCGG	117080	51.213726	36
GATCTCG	117020	51.075485	35
GCCGTAT	113325	51.014397	48
GTATCAT	107265	50.67927	51
AGATCTC	114945	50.67603	34
CTCGGTG	126470	50.427742	38
TCTCGGT	124150	50.324196	37
GGTCGCC	124235	49.836197	44
GTCGCCG	124675	49.688385	45
TAGATCT	116430	49.668953	33
TGGTCGC	118555	49.49606	43
CATTAAA	120810	49.461575	55
GTGGTCG	126930	48.962807	42
TCATTAA	118430	48.623295	54
GGTGGTC	128820	48.355843	41
ATCATTA	114160	48.348026	53